論文 - 詳細
| RRC ID | 6195 |
|---|---|
| 著者 | Vachutinsky Y, Oba M, Miyata K, Hiki S, Kano MR, Nishiyama N, Koyama H, Miyazono K, Kataoka K. |
| タイトル | Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles. |
| ジャーナル | J Control Release |
| Abstract |
Disulfide crosslinked polyplex micelles with RGD peptides were formed through ion complexation of thiolated c(RGDfK)-poly(ethylene glycol)-block-poly(L-lysine) (c(RGDfK)-PEG-P(Lys-SH)) and plasmid DNA encoding sFlt-1 and tested for their therapeutic effect in BxPC3 pancreatic adenocarcinoma tumor bearing mice. These micelles, systemically injected, demonstrated significant inhibition of tumor growth up to day 18, as a result of the antiangiogenic effect that was confirmed by vascular density measurements. Significant therapeutic activity of the 15% crosslinked micelle (c(RGDfK)-PEG-P(Lys-SH15)) was achieved by combined effect of increased tumor accumulation, interaction with endothelial cells and enhanced intracellular uptake through receptor-mediated endocytosis. These results suggest that RGD targeted crosslinked polyplex micelles can be effective plasmid DNA carriers for antiangiogenic gene therapy. |
| 巻・号 | 149(1) |
| ページ | 51-7 |
| 公開日 | 2011-1-5 |
| DOI | 10.1016/j.jconrel.2010.02.002 |
| PII | S0168-3659(10)00108-2 |
| PMID | 20138936 |
| MeSH | Animals Cell Line, Tumor Cross-Linking Reagents / chemistry DNA / administration & dosage* DNA / genetics Female Gene Transfer Techniques* Genetic Therapy* Injections, Intravenous Lysine / analogs & derivatives* Lysine / chemical synthesis Lysine / chemistry Mice Mice, Inbred BALB C Micelles Microvessels / drug effects Microvessels / ultrastructure Neoplasm Transplantation Neovascularization, Pathologic / genetics Neovascularization, Pathologic / pathology Neovascularization, Pathologic / prevention & control* Oligopeptides / chemistry Pancreatic Neoplasms / blood supply Pancreatic Neoplasms / genetics Pancreatic Neoplasms / pathology Pancreatic Neoplasms / therapy* Peptides, Cyclic / chemical synthesis Peptides, Cyclic / chemistry* Plasmids / administration & dosage Plasmids / genetics Polyethylene Glycols / chemical synthesis Polyethylene Glycols / chemistry* Polylysine / chemistry Reverse Transcriptase Polymerase Chain Reaction Sulfhydryl Compounds / chemistry Transfection Vascular Endothelial Growth Factor Receptor-1 / genetics* |
| IF | 7.727 |
| 引用数 | 64 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAcc-Luc+ (RDB02443) |