論文 - 詳細
| RRC ID | 61958 |
|---|---|
| 著者 | Ashida T, Sakai S, Taya M. |
| タイトル | Propagation of human iPS cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase. |
| ジャーナル | Artif Cells Nanomed Biotechnol |
| Abstract |
Cell encapsulation has been investigated as a bioproduction system in the biomedical and pharmaceutical fields. We encaps-ulated human induced pluripotent stem (hiPS) cells in duplex microcapsules prepared from an alginate derivative possessing phenolic hydroxyl moieties, in a single-step procedure based on two competing enzymatic reactions catalyzed by horseradish peroxidase (HRP) and catalase. The encapsulated cells maintained 91.4% viability and proliferated to fill the microcapsules following 19 days of culture. Encapsulated hiPS cells showed pluripotency comparable to that of unencapsulated cells during the cultures, as demonstrated by the expression of the SSEA-4 marker. |
| 巻・号 | 44(6) |
| ページ | 1406-9 |
| 公開日 | 2016-9-1 |
| DOI | 10.3109/21691401.2015.1029631 |
| PMID | 26148179 |
| MeSH | Alginates / chemistry* Animals Capsules Catalase / chemistry* Catalysis Cells, Immobilized / cytology Cells, Immobilized / metabolism Glucuronic Acid / chemistry Hexuronic Acids / chemistry Horseradish Peroxidase / chemistry Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Mice |
| IF | 3.343 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |