RRC ID 61966
Author Kato T, Miyagawa A, Kasuya MC, Ito A, Hatanaka K.
Title Purification by centrifugal partition chromatography of amphiphilic compounds, glycolipids and pseudo-glycolipids synthesized by using cells.
Journal J Chromatogr A
Abstract Centrifugal partition chromatography (CPC) was applied to separate amphiphilic glycolipids and pseudo-glycolipids synthesized by using cells. Neutral and acidic lipid fractions were isolated by CPC under suitable conditions respectively. Separation of neutral lipid, Gb3-type and Gb4-type oligosaccharide synthesized by using cells, was performed with a two-phase solvent system composed of chloroform-methanol-water at a volume ratio of 5:6:4. On the other hand, separation of acidic lipid, GM3-type oligosaccharide synthesized by using cells, and ganglioside extracted from rat brain were performed with a two-phase solvent system composed of butanol-ethanol-1% acetic acid at a volume ratio of 4:1:5. 8.3mg of Gb3 analogue, 5.1mg of Gb4 analogue, and 19.5mg of GM3 analogue were purified from 3.2l of culture medium obtained by incubation of African green-monkey kidney (Vero) cells with 50 microM n-dodecyl beta-lactoside using CPC.
Volume 1178(1-2)
Pages 154-9
Published 2008-1-18
DOI 10.1016/j.chroma.2007.11.094
PII S0021-9673(07)02078-X
PMID 18082172
MeSH Animals Brain Cell Line, Tumor Chlorocebus aethiops Chromatography / methods G(M3) Ganglioside / analysis G(M3) Ganglioside / isolation & purification* Gangliosides / analysis Gangliosides / isolation & purification* Glycolipids / analysis Glycolipids / isolation & purification* Mice Oligosaccharides / analysis Oligosaccharides / isolation & purification* Rats Vero Cells
IF 4.049
Resource
Human and Animal Cells Vero(RCB0001)