論文 - 詳細
| RRC ID | 61993 |
|---|---|
| 著者 | Mizushima R, Inoue K, Fujiwara H, Iwane AH, Watanabe TM, Kimura A. |
| タイトル | Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. |
| ジャーナル | Contrast Media Mol Imaging |
| Abstract |
Gas vesicle nanoparticles (GVs) are gas-containing protein assemblies expressed in bacteria and archaea. Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography. However, at present, the practical use of GVs is hampered by a lack of robust methodology for their induction into mammalian cells. Here, we demonstrate the genetic reconstitution of protein nanoparticles with characteristic bicone structures similar to natural GVs in a human breast cancer cell line KPL-4 and genetic control of their size and shape through expression of reduced sets of humanized gas vesicle genes cloned into Tol2 transposon vectors, referencing the natural gas vesicle gene clusters of the cyanobacteria planktothrix rubescens/agardhii. We then report the utility of these nanoparticles as multiplexed, sensitive, and genetically encoded contrast agents for hyperpolarized xenon chemical exchange saturation transfer (HyperCEST) MRI. |
| 巻・号 | 2020 |
| ページ | 5425934 |
| 公開日 | 2020-1-1 |
| DOI | 10.1155/2020/5425934 |
| PMID | 32256252 |
| PMC | PMC7091528 |
| MeSH | Bacterial Proteins / chemistry* Cell Line, Tumor Humans Magnetic Resonance Imaging* Nanoparticles / chemistry* Nanoparticles / ultrastructure Neoplasms / metabolism* Xenon Isotopes / chemistry* |
| IF | 2.651 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pT2b_mT_gvpC16-T2A-EGFP_pP_rm (RDB16904) pT2b_mT_gvpC20-T2A-mKO2_pP_rm (RDB16905) pT2b_mT_gvpC28-T2A-EGFP_pP_rm (RDB16906) |