| RRC ID |
61
|
| 著者 |
Sato Y, Igarashi Y, Hakamata Y, Murakami T, Kaneko T, Takahashi M, Seo N, Kobayashi E.
|
| タイトル |
Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Because serum albumin is specifically produced by mature hepatocytes, detection system of albumin producing cells could be a valuable tool to visualize liver regeneration or development. We have developed here an albumin enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having liver-specific reporter gene expression of red fluorescent protein. To study the transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver damage by repeated administration of CCl(4). DsRed2-positive cells were generated in the recipient liver after BMC injection. The number of transdifferentiated DsRed2-positive cells in chronic liver injury model was increased comparing with that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited to studying in vivo liver regeneration.
|
| 巻・号 |
311(2)
|
| ページ |
478-81
|
| 公開日 |
2003-11-14
|
| DOI |
10.1016/j.bbrc.2003.09.230
|
| PII |
S0006291X03020837
|
| PMID |
14592439
|
| MeSH |
Animals
Animals, Genetically Modified
Bone Marrow Transplantation*
Cell Transplantation
Hepatitis, Chronic / genetics
Hepatitis, Chronic / metabolism*
Hepatitis, Chronic / surgery
Liver / embryology*
Liver / metabolism*
Liver / surgery
Liver Regeneration*
Luminescent Proteins / genetics
Luminescent Proteins / metabolism*
Male
Models, Animal*
Rats / genetics
Rats, Wistar
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Serum Albumin / genetics
Serum Albumin / metabolism*
|
| IF |
2.985
|
| 引用数 |
34
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ラット |
W-Tg(Alb-DsRed2)34Jmsk(strainID=528)
W-Tg(Alb-DsRed2)42Jmsk(strainID=529) |
