論文 - 詳細
| RRC ID | 62012 |
|---|---|
| 著者 | Shimada H, Kuboshima M, Shiratori T, Nabeya Y, Takeuchi A, Takagi H, Nomura F, Takiguchi M, Ochiai T, Hiwasa T. |
| タイトル | Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma. |
| ジャーナル | Int J Oncol |
| Abstract |
In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E. coli was transformed with a lambdaZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified myomegalin (MMGL, phosphodiesterase 4D interacting protein/PDE4DIP) as a new SEREX antigen for esophageal SCC. Western blot analysis revealed that serum anti-myomegalin antibodies (s-MMGL-Abs) were present in 43 (47%) of 91 patients, but in only one (2.2%) of 45 healthy controls. Of the 21 patients with stage I disease, 8 (38%) were sero-positive. The positive rate of s-MMGL-Abs was greater than those of other conventional tumor markers. Reverse transcription-PCR analysis suggested that alternative splicing from myomegalin variant 1 to variant 5 may explain, in part, the development of s-MMGL-Abs. Although the presence of s-MMGL-Abs was not related to any clinicopathological features of the patients, multivariate analysis indicated that the presence of s-MMGL-Abs was significantly associated with a favorable prognosis. Consequently, s-MMGL-Abs may be a useful tumor marker to diagnose and establish a prognosis in patients with esophageal SCC. |
| 巻・号 | 30(1) |
| ページ | 97-103 |
| 公開日 | 2007-1-1 |
| PMID | 17143517 |
| MeSH | Aged Autoantibodies / blood* Biomarkers, Tumor / immunology Blotting, Western Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / immunology* Carcinoma, Squamous Cell / mortality Carcinoma, Squamous Cell / pathology DNA Primers DNA, Complementary / genetics Esophageal Neoplasms / genetics Esophageal Neoplasms / immunology* Esophageal Neoplasms / mortality Esophageal Neoplasms / pathology Female Humans Male Microtubule-Associated Proteins / blood Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / immunology Middle Aged Reverse Transcriptase Polymerase Chain Reaction Survival Analysis |
| IF | 3.899 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | WI-38(RCB0702) |