| RRC ID |
62012
|
| 著者 |
Shimada H, Kuboshima M, Shiratori T, Nabeya Y, Takeuchi A, Takagi H, Nomura F, Takiguchi M, Ochiai T, Hiwasa T.
|
| タイトル |
Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E. coli was transformed with a lambdaZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified myomegalin (MMGL, phosphodiesterase 4D interacting protein/PDE4DIP) as a new SEREX antigen for esophageal SCC. Western blot analysis revealed that serum anti-myomegalin antibodies (s-MMGL-Abs) were present in 43 (47%) of 91 patients, but in only one (2.2%) of 45 healthy controls. Of the 21 patients with stage I disease, 8 (38%) were sero-positive. The positive rate of s-MMGL-Abs was greater than those of other conventional tumor markers. Reverse transcription-PCR analysis suggested that alternative splicing from myomegalin variant 1 to variant 5 may explain, in part, the development of s-MMGL-Abs. Although the presence of s-MMGL-Abs was not related to any clinicopathological features of the patients, multivariate analysis indicated that the presence of s-MMGL-Abs was significantly associated with a favorable prognosis. Consequently, s-MMGL-Abs may be a useful tumor marker to diagnose and establish a prognosis in patients with esophageal SCC.
|
| 巻・号 |
30(1)
|
| ページ |
97-103
|
| 公開日 |
2007-1-1
|
| PMID |
17143517
|
| MeSH |
Aged
Autoantibodies / blood*
Biomarkers, Tumor / immunology
Blotting, Western
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / immunology*
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology
DNA Primers
DNA, Complementary / genetics
Esophageal Neoplasms / genetics
Esophageal Neoplasms / immunology*
Esophageal Neoplasms / mortality
Esophageal Neoplasms / pathology
Female
Humans
Male
Microtubule-Associated Proteins / blood
Microtubule-Associated Proteins / genetics*
Microtubule-Associated Proteins / immunology
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
WI-38(RCB0702) |
