RRC ID 62012
Author Shimada H, Kuboshima M, Shiratori T, Nabeya Y, Takeuchi A, Takagi H, Nomura F, Takiguchi M, Ochiai T, Hiwasa T.
Title Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma.
Journal Int J Oncol
Abstract In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E. coli was transformed with a lambdaZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified myomegalin (MMGL, phosphodiesterase 4D interacting protein/PDE4DIP) as a new SEREX antigen for esophageal SCC. Western blot analysis revealed that serum anti-myomegalin antibodies (s-MMGL-Abs) were present in 43 (47%) of 91 patients, but in only one (2.2%) of 45 healthy controls. Of the 21 patients with stage I disease, 8 (38%) were sero-positive. The positive rate of s-MMGL-Abs was greater than those of other conventional tumor markers. Reverse transcription-PCR analysis suggested that alternative splicing from myomegalin variant 1 to variant 5 may explain, in part, the development of s-MMGL-Abs. Although the presence of s-MMGL-Abs was not related to any clinicopathological features of the patients, multivariate analysis indicated that the presence of s-MMGL-Abs was significantly associated with a favorable prognosis. Consequently, s-MMGL-Abs may be a useful tumor marker to diagnose and establish a prognosis in patients with esophageal SCC.
Volume 30(1)
Pages 97-103
Published 2007-1-1
PMID 17143517
MeSH Aged Autoantibodies / blood* Biomarkers, Tumor / immunology Blotting, Western Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / immunology* Carcinoma, Squamous Cell / mortality Carcinoma, Squamous Cell / pathology DNA Primers DNA, Complementary / genetics Esophageal Neoplasms / genetics Esophageal Neoplasms / immunology* Esophageal Neoplasms / mortality Esophageal Neoplasms / pathology Female Humans Male Microtubule-Associated Proteins / blood Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / immunology Middle Aged Reverse Transcriptase Polymerase Chain Reaction Survival Analysis
IF 3.899
Human and Animal Cells WI-38(RCB0702)