RRC ID 62017
Author Tani H, Okuda S, Nakamura K, Aoki M, Umemura T.
Title Short-lived long non-coding RNAs as surrogate indicators for chemical exposure and LINC00152 and MALAT1 modulate their neighboring genes.
Journal PLoS One
Abstract Whole transcriptome analyses have revealed a large number of novel long non-coding RNAs (lncRNAs). Although accumulating evidence demonstrates that lncRNAs play important roles in regulating gene expression, the detailed mechanisms of action of most lncRNAs remain unclear. We previously reported that a novel class of lncRNAs with a short half-life (t1/2 < 4 h) in HeLa cells, termed short-lived non-coding transcripts (SLiTs), are closely associated with physiological and pathological functions. In this study, we focused on 26 SLiTs and nuclear-enriched abundant lncRNA, MALAT1(t1/2 of 7.6 h in HeLa cells) in neural stem cells (NSCs) derived from human induced pluripotent stem cells, and identified four SLiTs (TUG1, GAS5, FAM222-AS1, and SNHG15) that were affected by the following typical chemical stresses (oxidative stress, heavy metal stress and protein synthesis stress). We also found the expression levels of LINC00152 (t1/2 of 2.1 h in NSCs), MALAT1 (t1/2 of 1.8 h in NSCs), and their neighboring genes were elevated proportionally to the chemical doses. Moreover, we confirmed that the overexpression of LINC00152 or MALAT1 upregulated the expressions of their neighboring genes even in the absence of chemical stress. These results reveal that LINC00152 and MALAT1 modulate their neighboring genes, and thus provide a deeper understanding of the functions of lncRNAs.
Volume 12(7)
Pages e0181628
Published 2017-1-1
DOI 10.1371/journal.pone.0181628
PII PONE-D-17-20884
PMID 28719640
PMC PMC5515456
MeSH Cell Differentiation / drug effects Cell Differentiation / genetics Cell Line Environmental Pollutants / pharmacology* HeLa Cells Humans Induced Pluripotent Stem Cells / cytology Neural Stem Cells / cytology Neural Stem Cells / drug effects Neural Stem Cells / metabolism RNA Stability / drug effects RNA Stability / genetics RNA, Long Noncoding / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Time Factors Transcription, Genetic / drug effects
IF 2.74
Human and Animal Cells 201B7(HPS0063)