RRC ID 62020
Author Yakop F, Abd Ghafar SA, Yong YK, Saiful Yazan L, Mohamad Hanafiah R, Lim V, Eshak Z.
Title Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines.
Journal Artif Cells Nanomed Biotechnol
Abstract PURPOSE:The purpose of this study was to investigate apoptotic activity of silver nanoparticle Clinacanthus nutans (AgNps-CN) towards HSC-4 cell lines (oral squamous cell carcinoma cell lines).
METHODS:Methods involved were MTT assay (cytotoxic activity), morphological cells analysis, flow cytometry and cell cycle analysis and western blot.
RESULTS:MTT assay revealed IC50 concentration was 1.61 µg/mL, 3T3-L1 cell lines were used to determine whether AgNps-CN is cytotoxic to normal cells. At the highest concentration (3 µg/mL), no cytotoxic activity has been observed. Flow cytometry assay revealed AgNps-CN caused apoptosis effects towards HSC-4 cell lines with significant changes were observed at G1 phase when compared with untreated cells. Morphological cells analysis revealed that most of the cells exhibit apoptosis characteristics rather than necrosis. Protein study revealed that ratio of Bax/Bcl-2 increased mainly due to down-regulation of Bcl-2 expression.
CONCLUSION:AgNps-CN have shown potential in inhibiting HSC-4 cell lines. IC50 was low compared to few studies involving biosynthesized of silver nanoparticles. Apoptosis effects were shown towards HSC-4 cell lines by the increased in Bax/Bcl-2 protein ratio. Further study such as PCR or in vivo studies are required.
Volume 46(sup2)
Pages 131-139
Published 2018-1-1
DOI 10.1080/21691401.2018.1452750
PMID 29561182
MeSH 3T3 Cells Acanthaceae / chemistry* Animals Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology Apoptosis / drug effects* Carcinoma, Squamous Cell / pathology* Cell Cycle Checkpoints / drug effects Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Humans Metal Nanoparticles / chemistry* Mice Mouth Neoplasms / pathology* Nanotechnology Plant Extracts / chemistry Plant Leaves / chemistry* Silver / chemistry Silver / pharmacology*
IF 3.343
Human and Animal Cells HSC-4(RCB1902)