| RRC ID |
62047
|
| 著者 |
Matsui Y, Inomata M, Tojigamori M, Sonoda K, Shiraishi N, Kitano S.
|
| タイトル |
Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
New modalities are necessary for the treatment of patients with unresectable gastric cancer. The aim of this study was to investigate whether or not anti-HER2 antibody could suppress the growth of human gastric cancer cells with HER2 overexpression in vitro and in vivo. Four human gastric cancer cell lines, NCI-N87, MKN-45P, Kato-III, and MKN-1, were used in this study. The suppression of cell proliferation in vitro and of subcutaneous tumor growth in a nude mouse model after treatment with trastuzumab was examined. The expression of HER2 protein was investigated by Western blot analysis. The effect of trastuzumab on the survival rate of nude mice with peritoneal dissemination was examined. Trastuzumab significantly reduced proliferative activity in NCI-N87, a HER2-overexpressing human gastric cancer cell line, in vitro. In the nude mouse model with transplanted subcutaneous tumor, trastuzumab significantly suppressed the tumor growth of NCI-N87 cells, and then HER2 expression was reduced. Trastuzumab improved the survival rate of mice with peritoneal dissemination of MKN-45P cells. Trastuzumab therapy is a potential candidate for a novel treatment of HER2-overexpressing gastric cancer.
|
| 巻・号 |
27(3)
|
| ページ |
681-5
|
| 公開日 |
2005-9-1
|
| PMID |
16077916
|
| MeSH |
Animals
Antibodies, Monoclonal / pharmacology
Antibodies, Monoclonal / therapeutic use*
Antibodies, Monoclonal, Humanized
Ascites / pathology
Ascites / prevention & control
Blotting, Western
Cell Line, Tumor
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Female
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Peritoneum / drug effects
Peritoneum / pathology
Receptor, ErbB-2 / biosynthesis*
Receptor, ErbB-2 / immunology
Stomach Neoplasms / mortality
Stomach Neoplasms / pathology
Stomach Neoplasms / prevention & control*
Survival Analysis
Survival Rate
Time Factors
Trastuzumab
Treatment Outcome
Xenograft Model Antitumor Assays / methods*
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003) |
