RRC ID 62049
著者 Terauchi M, Ikeda G, Nishida K, Tamura A, Yamaguchi S, Harada K, Yui N.
タイトル Supramolecular Polyelectrolyte Complexes of Bone Morphogenetic Protein-2 with Sulfonated Polyrotaxanes to Induce Enhanced Osteogenic Differentiation.
ジャーナル Macromol Biosci
Abstract Although bone morphogenetic protein-2 (BMP-2) has received considerable attention because of its strong osteoinductivity, the clinical application of BMP-2 is limited due to its degradation and deactivation under physiological conditions. Negatively charged heparin is known to form polyelectrolyte complexes with BMP-2 to prevent deactivation and enhance the osteoinduction capability of BMP-2. Herein, we report the sulfonated polyrotaxanes (S-PRX) composed of α-cyclodextrin threaded onto a linear polymer for the protection of BMP-2 through the polyelectrolyte complex formation. When MC3T3-E1 osteoprogenitor cells were treated with the S-PRX/BMP-2 complexes, significantly high alkaline phosphatase production and mineralized matrix deposition were observed compared with that of free BMP-2 and heparin/BMP-2 complexes. Note that the S-PRXs showed negligible anticoagulant activity and cytotoxicity, whereas heparin showed strong anticoagulant activity. Accordingly, the S-PRXs are promising candidates for enhanced osteoinduction ability of BMP-2 without toxicity and anticoagulant activity and could contribute to clinical bone regeneration.
巻・号 15(7)
ページ 953-64
公開日 2015-7-1
DOI 10.1002/mabi.201500032
PMID 25810046
MeSH Bone Morphogenetic Protein 2 / chemistry* Bone Morphogenetic Protein 2 / pharmacology Bone Regeneration / drug effects* Humans Osteogenesis / drug effects* Rotaxanes / chemistry* Rotaxanes / pharmacology alpha-Cyclodextrins
IF 3.416
リソース情報
ヒト・動物細胞 C2C12(RCB0987) MC3T3-E1(RCB1126)