論文 - 詳細
| RRC ID | 62049 |
|---|---|
| 著者 | Terauchi M, Ikeda G, Nishida K, Tamura A, Yamaguchi S, Harada K, Yui N. |
| タイトル | Supramolecular Polyelectrolyte Complexes of Bone Morphogenetic Protein-2 with Sulfonated Polyrotaxanes to Induce Enhanced Osteogenic Differentiation. |
| ジャーナル | Macromol Biosci |
| Abstract |
Although bone morphogenetic protein-2 (BMP-2) has received considerable attention because of its strong osteoinductivity, the clinical application of BMP-2 is limited due to its degradation and deactivation under physiological conditions. Negatively charged heparin is known to form polyelectrolyte complexes with BMP-2 to prevent deactivation and enhance the osteoinduction capability of BMP-2. Herein, we report the sulfonated polyrotaxanes (S-PRX) composed of α-cyclodextrin threaded onto a linear polymer for the protection of BMP-2 through the polyelectrolyte complex formation. When MC3T3-E1 osteoprogenitor cells were treated with the S-PRX/BMP-2 complexes, significantly high alkaline phosphatase production and mineralized matrix deposition were observed compared with that of free BMP-2 and heparin/BMP-2 complexes. Note that the S-PRXs showed negligible anticoagulant activity and cytotoxicity, whereas heparin showed strong anticoagulant activity. Accordingly, the S-PRXs are promising candidates for enhanced osteoinduction ability of BMP-2 without toxicity and anticoagulant activity and could contribute to clinical bone regeneration. |
| 巻・号 | 15(7) |
| ページ | 953-64 |
| 公開日 | 2015-7-1 |
| DOI | 10.1002/mabi.201500032 |
| PMID | 25810046 |
| MeSH | Bone Morphogenetic Protein 2 / chemistry* Bone Morphogenetic Protein 2 / pharmacology Bone Regeneration / drug effects* Humans Osteogenesis / drug effects* Rotaxanes / chemistry* Rotaxanes / pharmacology alpha-Cyclodextrins |
| IF | 3.416 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) MC3T3-E1(RCB1126) |