RRC ID |
62049
|
著者 |
Terauchi M, Ikeda G, Nishida K, Tamura A, Yamaguchi S, Harada K, Yui N.
|
タイトル |
Supramolecular Polyelectrolyte Complexes of Bone Morphogenetic Protein-2 with Sulfonated Polyrotaxanes to Induce Enhanced Osteogenic Differentiation.
|
ジャーナル |
Macromol Biosci
|
Abstract |
Although bone morphogenetic protein-2 (BMP-2) has received considerable attention because of its strong osteoinductivity, the clinical application of BMP-2 is limited due to its degradation and deactivation under physiological conditions. Negatively charged heparin is known to form polyelectrolyte complexes with BMP-2 to prevent deactivation and enhance the osteoinduction capability of BMP-2. Herein, we report the sulfonated polyrotaxanes (S-PRX) composed of α-cyclodextrin threaded onto a linear polymer for the protection of BMP-2 through the polyelectrolyte complex formation. When MC3T3-E1 osteoprogenitor cells were treated with the S-PRX/BMP-2 complexes, significantly high alkaline phosphatase production and mineralized matrix deposition were observed compared with that of free BMP-2 and heparin/BMP-2 complexes. Note that the S-PRXs showed negligible anticoagulant activity and cytotoxicity, whereas heparin showed strong anticoagulant activity. Accordingly, the S-PRXs are promising candidates for enhanced osteoinduction ability of BMP-2 without toxicity and anticoagulant activity and could contribute to clinical bone regeneration.
|
巻・号 |
15(7)
|
ページ |
953-64
|
公開日 |
2015-7-1
|
DOI |
10.1002/mabi.201500032
|
PMID |
25810046
|
MeSH |
Bone Morphogenetic Protein 2 / chemistry*
Bone Morphogenetic Protein 2 / pharmacology
Bone Regeneration / drug effects*
Humans
Osteogenesis / drug effects*
Rotaxanes / chemistry*
Rotaxanes / pharmacology
alpha-Cyclodextrins
|
IF |
3.416
|
リソース情報 |
ヒト・動物細胞 |
C2C12(RCB0987)
MC3T3-E1(RCB1126) |