Reference - Detail
|Author||Kaneda M, Odaka T, Suetake H, Tahara D, Miyadai T.|
|Title||Teleost IL-6 promotes antibody production through STAT3 signaling via IL-6R and gp130.|
|Journal||Dev Comp Immunol|
Teleost IL-6 is upregulated after antigen stimulation; therefore, we hypothesized that fish IL-6 contributes to antibody production during immune responses against infections. To verify this hypothesis, we first cloned IL-6R and gp130 in fugu (Takifugu rubripes) in the present study. The membrane and soluble forms of IL-6R were identified by the identification of cDNA clones of IL-6R homologues. Three STAT3-docking sites were found in the intracellular region of fugu gp130. Expression analysis showed that fugu IL-6R and gp130 were expressed in mIgM(+) B cells, suggesting that fugu B cells are stimulated by IL-6. Recombinant fugu IL-6 (rfIL-6) increased the gene expression of secretory antibodies by mIgM(+) B cells in vitro. The rfIL-6 and soluble form of rfIL-6R activated STAT3 phosphorylation in the B cells and a cultured cell line transfected with fugu gp130. These results indicate that fugu IL-6 enhances antibody production in the B-cell lineage via gp130 and STAT3 signaling.
|MeSH||Amino Acid Sequence Animals Antibody Formation* B-Lymphocytes / metabolism Cloning, Molecular Cytokine Receptor gp130 / metabolism* Fish Proteins / metabolism* Humans Interleukin-6 / immunology* Molecular Sequence Data Phylogeny Receptors, Interleukin-6 / metabolism* STAT3 Transcription Factor / metabolism* Sequence Alignment Signal Transduction* Takifugu / immunology*|
|Human and Animal Cells||Ba/F3(RCB0805)|