論文 - 詳細
RRC ID | 62057 |
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著者 | Kaneda M, Odaka T, Suetake H, Tahara D, Miyadai T. |
タイトル | Teleost IL-6 promotes antibody production through STAT3 signaling via IL-6R and gp130. |
ジャーナル | Dev Comp Immunol |
Abstract |
Teleost IL-6 is upregulated after antigen stimulation; therefore, we hypothesized that fish IL-6 contributes to antibody production during immune responses against infections. To verify this hypothesis, we first cloned IL-6R and gp130 in fugu (Takifugu rubripes) in the present study. The membrane and soluble forms of IL-6R were identified by the identification of cDNA clones of IL-6R homologues. Three STAT3-docking sites were found in the intracellular region of fugu gp130. Expression analysis showed that fugu IL-6R and gp130 were expressed in mIgM(+) B cells, suggesting that fugu B cells are stimulated by IL-6. Recombinant fugu IL-6 (rfIL-6) increased the gene expression of secretory antibodies by mIgM(+) B cells in vitro. The rfIL-6 and soluble form of rfIL-6R activated STAT3 phosphorylation in the B cells and a cultured cell line transfected with fugu gp130. These results indicate that fugu IL-6 enhances antibody production in the B-cell lineage via gp130 and STAT3 signaling. |
巻・号 | 38(2) |
ページ | 224-31 |
公開日 | 2012-10-1 |
DOI | 10.1016/j.dci.2012.02.002 |
PII | S0145-305X(12)00025-0 |
PMID | 22469658 |
MeSH | Amino Acid Sequence Animals Antibody Formation* B-Lymphocytes / metabolism Cloning, Molecular Cytokine Receptor gp130 / metabolism* Fish Proteins / metabolism* Humans Interleukin-6 / immunology* Molecular Sequence Data Phylogeny Receptors, Interleukin-6 / metabolism* STAT3 Transcription Factor / metabolism* Sequence Alignment Signal Transduction* Takifugu / immunology* |
IF | 3.192 |
リソース情報 | |
ヒト・動物細胞 | Ba/F3(RCB0805) |