論文 - 詳細
| RRC ID | 62063 |
|---|---|
| 著者 | Zheng Z, Zhang L, Kong L, Wang A, Gong Y, Zhang X. |
| タイトル | The behavior of MC3T3-E1 cells on chitosan/poly-L-lysine composite films: effect of nanotopography, surface chemistry, and wettability. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
In the present work, a series of composite films were produced from chitosan/poly-L-lysine blend solutions. The surface topography, chemistry, and wettability of composite films were characterized by atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and contact angle assay, respectively. For all composite films, blending with poly-L-lysine induced changes in surface chemistry and wettability. Interestingly, it was also found that increasing poly-L-lysine weight fraction in blend solutions could result in different nanoscaled surface topographic features, which displayed particle-, granule-, or fiber-dominant morphologies. MC3T3-E1 osteoblast-like cells were cultured on all composite films to evaluate the effects of surface nanotopography, chemistry, and wettability on cell behavior. The observations indicated that MC3T3-E1 cell behavior was affected by surface topography, chemistry, and wettability simultaneously and that cells showed strong responses to surface topography. On fiber-dominant surface, cells fully spread with obvious cytoskeleton organization and exhibited significantly higher level of adhesion and proliferation compared with particle- or granule-dominant surfaces. Furthermore, fiber-dominant surface also induced greater expression of mature osteogenic marker osteocalcin and higher mineralization based on RT-PCR and von Kossa staining. The results suggest that topographic modification of chitosan substratum at the nanoscale may be exploited in regulating cell behavior for its applications in tissue engineering. |
| 巻・号 | 89(2) |
| ページ | 453-65 |
| 公開日 | 2009-5-1 |
| DOI | 10.1002/jbm.a.31979 |
| PMID | 18431777 |
| MeSH | Animals Calcification, Physiologic / drug effects Cell Adhesion / drug effects Cell Nucleus / drug effects Cell Nucleus / metabolism Cell Proliferation / drug effects Cell Shape / drug effects Chitosan / pharmacology* Cytoskeleton / drug effects Cytoskeleton / metabolism Gene Expression Regulation / drug effects Materials Testing* Mice Microscopy, Atomic Force Nanocomposites / chemistry* Osteoblasts / cytology* Osteoblasts / drug effects* Osteocalcin / genetics Osteocalcin / metabolism Polylysine / pharmacology* Spectroscopy, Fourier Transform Infrared Surface Properties / drug effects Water Wettability / drug effects |
| IF | 3.525 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |