RRC ID 62066
Author Nagasato AI, Yamashita H, Matsuo M, Ueda K, Kioka N.
Title The distribution of vinculin to lipid rafts plays an important role in sensing stiffness of extracellular matrix.
Journal Biosci Biotechnol Biochem
Abstract Extracellular matrix (ECM) stiffness regulates cell differentiation, survival, and migration. Our previous study has shown that the interaction of the focal adhesion protein vinculin with vinexin α plays a critical role in sensing ECM stiffness and regulating stiffness-dependent cell migration. However, the mechanism how vinculin-vinexin α interaction affects stiffness-dependent cell migration is unclear. Lipid rafts are membrane microdomains that are known to affect ECM-induced signals and cell behaviors. Here, we show that vinculin and vinexin α can localize to lipid rafts. Cell-ECM adhesion, intracellular tension, and a rigid ECM promote vinculin distribution to lipid rafts. The disruption of lipid rafts with Methyl-β-cyclodextrin impaired the ECM stiffness-mediated regulation of vinculin behavior and rapid cell migration on rigid ECM. These results indicate that lipid rafts play an important role in ECM-stiffness regulation of cell migration via vinculin.
Volume 81(6)
Pages 1136-1147
Published 2017-6-1
DOI 10.1080/09168451.2017.1289074
PMID 28485208
MeSH Animals Biomechanical Phenomena Caveolin 1 / genetics Caveolin 1 / metabolism Cell Adhesion / drug effects Cell Line Cell Movement / drug effects Embryo, Mammalian Extracellular Matrix / drug effects Extracellular Matrix / metabolism* Extracellular Matrix / ultrastructure Fibroblasts / drug effects Fibroblasts / metabolism* Fibroblasts / ultrastructure Focal Adhesions / drug effects Focal Adhesions / metabolism* Focal Adhesions / ultrastructure Gene Expression Regulation Hardness Membrane Microdomains / drug effects Membrane Microdomains / metabolism* Membrane Microdomains / ultrastructure Membrane Proteins / genetics Membrane Proteins / metabolism Mice Muscle Proteins / genetics Muscle Proteins / metabolism* Paxillin / genetics Paxillin / metabolism Protein Binding Protein Isoforms / genetics Protein Isoforms / metabolism Signal Transduction Vinculin / genetics Vinculin / metabolism* beta-Cyclodextrins / pharmacology
IF 1.516
Human and Animal Cells 293T(RCB2202)