Reference - Detail
|Author||Nagasato AI, Yamashita H, Matsuo M, Ueda K, Kioka N.|
|Title||The distribution of vinculin to lipid rafts plays an important role in sensing stiffness of extracellular matrix.|
|Journal||Biosci Biotechnol Biochem|
Extracellular matrix (ECM) stiffness regulates cell differentiation, survival, and migration. Our previous study has shown that the interaction of the focal adhesion protein vinculin with vinexin α plays a critical role in sensing ECM stiffness and regulating stiffness-dependent cell migration. However, the mechanism how vinculin-vinexin α interaction affects stiffness-dependent cell migration is unclear. Lipid rafts are membrane microdomains that are known to affect ECM-induced signals and cell behaviors. Here, we show that vinculin and vinexin α can localize to lipid rafts. Cell-ECM adhesion, intracellular tension, and a rigid ECM promote vinculin distribution to lipid rafts. The disruption of lipid rafts with Methyl-β-cyclodextrin impaired the ECM stiffness-mediated regulation of vinculin behavior and rapid cell migration on rigid ECM. These results indicate that lipid rafts play an important role in ECM-stiffness regulation of cell migration via vinculin.
|MeSH||Animals Biomechanical Phenomena Caveolin 1 / genetics Caveolin 1 / metabolism Cell Adhesion / drug effects Cell Line Cell Movement / drug effects Embryo, Mammalian Extracellular Matrix / drug effects Extracellular Matrix / metabolism* Extracellular Matrix / ultrastructure Fibroblasts / drug effects Fibroblasts / metabolism* Fibroblasts / ultrastructure Focal Adhesions / drug effects Focal Adhesions / metabolism* Focal Adhesions / ultrastructure Gene Expression Regulation Hardness Membrane Microdomains / drug effects Membrane Microdomains / metabolism* Membrane Microdomains / ultrastructure Membrane Proteins / genetics Membrane Proteins / metabolism Mice Muscle Proteins / genetics Muscle Proteins / metabolism* Paxillin / genetics Paxillin / metabolism Protein Binding Protein Isoforms / genetics Protein Isoforms / metabolism Signal Transduction Vinculin / genetics Vinculin / metabolism* beta-Cyclodextrins / pharmacology|
|Human and Animal Cells||293T(RCB2202)|