RRC ID 62070
著者 Buchegger K, Silva R, López J, Ili C, Araya JC, Leal P, Brebi P, Riquelme I, Roa JC.
タイトル The ERK/MAPK pathway is overexpressed and activated in gallbladder cancer.
ジャーナル Pathol Res Pract
Abstract Gallbladder cancer (GBC) is a highly fatal disease with poor prognosis and few therapeutic alternatives. Molecular profiling has revealed that the deregulation in the ERK/MAPK signaling pathway plays a crucial role in many disease and malignancies, including GBC. The aim of this study was to measure the expression of ERK1/2 and p-ERK1/2 in a population with high GBC-related mortality, such as the Chilean population, and characterize the protein expression of this ERK/MAPK pathway in seven GBC cell lines. Immunohistochemistry (IHC) for ERK1/2 and p-ERK1/2 was performed in 123 GBC tissues and 37 chronic cholecystitis (CC) tissues. In addition, protein expression analysis by western blot for ERK1/2, p-ERK1/2, EGFR, ERBB2 and ERBB3 were performed in seven GBC cell lines (GB-d1, G415, NOZ, OCUG-1, TGBC-1, TGBC-2 and TGBC-24). A higher ERK1/2 and p-ERK1/2 expression was found in GBC tissues compared to chronic cholecystitis (CC) tissues (P<0.001). However, neither significant differences in overall survival nor significant associations with any of the clinicopathological features were found by comparing low and high expression of both ERK1/2 and p-ERK1/2. Western blot analysis of seven GBC cell lines showed that, in general, GB-d1, G415 and NOZ cells evidenced a strong expression of ERK1/2, p-ERK1/2, EGFR, ERBB2 and ERBB3. Therefore, ERK1/2 and p-ERK1/2 seem to be important in the development of GBC and GB-d1, G415 and NOZ cell lines may be used as experimental models for further in vitro and in vivo studies that help to decipher the role of MAPK/ERK pathway in gallbladder carcinogenesis.
巻・号 213(5)
ページ 476-482
公開日 2017-5-1
DOI 10.1016/j.prp.2017.01.025
PII S0344-0338(16)30650-1
PMID 28285962
MeSH Adult Aged Aged, 80 and over Cell Line, Tumor Female Gallbladder / metabolism Gallbladder / pathology Gallbladder Neoplasms / metabolism* Gallbladder Neoplasms / pathology Gene Expression Regulation, Neoplastic* Humans Immunohistochemistry MAP Kinase Signaling System* Male Middle Aged Mitogen-Activated Protein Kinase 1 / metabolism* Mitogen-Activated Protein Kinase 3 / metabolism* Signal Transduction* Tissue Array Analysis Young Adult
IF 2.05
リソース情報
ヒト・動物細胞 TGBC1TKB(RCB1129) TGBC2TKB(RCB1130)