| RRC ID |
62078
|
| Author |
Yoshii Y, Waki A, Yoshida K, Kakezuka A, Kobayashi M, Namiki H, Kuroda Y, Kiyono Y, Yoshii H, Furukawa T, Asai T, Okazawa H, Gelovani JG, Fujibayashi Y.
|
| Title |
The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation.
|
| Journal |
Biomaterials
|
| Abstract |
Two-dimensional (2D) cell cultures are essential for drug development and tumor research. However, the limitations of 2D cultures are widely recognized, and a better technique is needed. Recent studies have indicated that a strong physical contact between cells and 2D substrates induces cellular characteristics that differ from those of tumors growing in vivo. 3D cell cultures using various substrates are then developing; nevertheless, conventional approaches have failed in maintenance of cellular proliferation and viability, uniformity, reproducibility, and/or simplicity of these assays. Here, we developed a 3D culture system with inorganic nanoscale scaffolding using nanoimprinting technology (nano-culture plates), which reproduced the characteristics of tumor cells growing in vivo. Diminished cell-to-substrate physical contact facilitated spontaneous tumor cell migration, intercellular adhesion, and multi-cellular 3D-spheroid formation while maintaining cellular proliferation and viability. The resulting multi-cellular spheroids formed hypoxic core regions similar to tumors growing in vivo. This technology allows creating uniform and highly-reproducible 3D cultures, which is easily applicable for microscopic and spectrophotometric assays, which can be used for high-throughput/high-content screening of anticancer drugs and should accelerate discovery of more effective anticancer therapies.
|
| Volume |
32(26)
|
| Pages |
6052-8
|
| Published |
2011-9-1
|
| DOI |
10.1016/j.biomaterials.2011.04.076
|
| PII |
S0142-9612(11)00498-4
|
| PMID |
21640378
|
| MeSH |
Animals
Cell Culture Techniques / methods*
Cell Line, Tumor
Cell Movement / physiology
Cell Proliferation
Cell Survival / physiology
Humans
Mice
Spheroids, Cellular / cytology*
Tissue Scaffolds*
|
| IF |
10.317
|
| Resource |
| Human and Animal Cells |
Li-7(RCB1941)
A549(RCB0098)
RBE(RCB1292) |
