| RRC ID |
62084
|
| Author |
Chen RI, Gallant ND, Smith JR, Kipper MJ, Simon CG Jr.
|
| Title |
Time-dependent effects of pre-aging polymer films in cell culture medium on cell adhesion and spreading.
|
| Journal |
J Mater Sci Mater Med
|
| Abstract |
We have tested the hypothesis that cell adhesion and spreading on polymer films are influenced by the amount of time that the polymer films are pre-aged in cell culture medium. Cell adhesion and spreading were assessed after a 6-h culture on poly(D,L-lactic acid) (PDLLA) films that had been pre-aged in cell culture medium for 30 min, 1, 3 or 7 d. Cell adhesion and spread area were enhanced as the duration of pre-aging PDLLA films in cell culture medium was increased. Materials characterization showed that the hydrophobicity and surface morphology of the PDLLA films changed with increasing length of pre-aging time. These results suggest that cell adhesion and spreading are sensitive to the time-dependent changes in PDLLA hydrophobicity and surface morphology that occur during exposure of the polymer to cell medium for different lengths of time. These results demonstrate that cell response to a degradable, biomedical polymer can change as a function of the amount of time that the polymer is exposed to physiological medium.
|
| Volume |
19(4)
|
| Pages |
1759-66
|
| Published |
2008-4-1
|
| DOI |
10.1007/s10856-007-3309-x
|
| PMID |
18049876
|
| MeSH |
Animals
Automation
Biocompatible Materials / chemistry*
Cell Adhesion
Cell Culture Techniques / instrumentation*
Cell Culture Techniques / methods
Cell Line
Culture Media* / metabolism
Lactic Acid / chemistry
Mice
Microscopy, Atomic Force
Microscopy, Fluorescence
Models, Biological
Polyesters
Polymers / chemistry*
Time Factors
Vinculin / chemistry
|
| IF |
2.489
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
