RRC ID 62089
著者 Seino S, Matsuoka R, Masuda Y, Kunou M, Okada Y, Saika S.
タイトル Topical hyaluronan alone promotes corneal epithelial cell migration whereas combination with benzalkonium chloride impairs epithelial wound healing.
ジャーナル Cutan Ocul Toxicol
Abstract Purpose: To evaluate the effects of topical hyaluronan (HA) on corneal epithelial wound healing when administered with or without benzalkonium chloride (BAC).Methods: A cultured human corneal epithelial cell line (HCE-T) was subjected to in vitro scratch assays and in situ epithelial migration was evaluated in organ-cultured rabbit corneas. The corneal epithelium of C57BL/6J mice was also evaluated to determine in vivo wound healing. An in vivo imaging system was also used to evaluate the effects of HA on eye drop retention on the ocular surface.Results: The findings revealed the promotion of HCE-T migration, in situ rabbit corneal epithelial migration, and in vivo wound healing in mouse corneal epithelium by HA. Pre-treatment with HA also protected against delayed epithelial wound healing in BAC in vitro. However, pre-treatment with 3 mg/mL HA did not show a protective effect against BAC in vivo, but instead delayed epithelial wound healing and increased detection of cleaved caspase-3. This suggested that HA promotes the retention of BAC on the ocular surface. The instilled HA was retained after 15 min, at a significantly higher rate than for phosphate-buffered saline.Conclusions: The combination of HA and BAC impaired wound healing in the corneal epithelium.
巻・号 39(1)
ページ 13-20
公開日 2020-3-1
DOI 10.1080/15569527.2019.1673402
PMID 31588814
MeSH Animals Benzalkonium Compounds / administration & dosage* Benzalkonium Compounds / adverse effects Cell Line Cell Movement / drug effects* Cornea / cytology* Cornea / drug effects* Epithelial Cells / drug effects* Humans Hyaluronic Acid / adverse effects Hyaluronic Acid / pharmacology* Mice Ophthalmic Solutions Rabbits Tissue Culture Techniques Wound Healing / drug effects
IF 1.385
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)