論文 - 詳細
| RRC ID | 62090 |
|---|---|
| 著者 | Sakuma S, Kumagai H, Shimosato M, Kitamura T, Mohri K, Ikejima T, Hiwatari K, Koike S, Tobita E, McClure R, Gore JC, Pham W. |
| タイトル | Toxicity studies of coumarin 6-encapsulated polystyrene nanospheres conjugated with peanut agglutinin and poly(N-vinylacetamide) as a colonoscopic imaging agent in rats. |
| ジャーナル | Nanomedicine |
| Abstract |
UNLABELLED:We are investigating an imaging agent that detects early-stage primary colorectal cancer on the mucosal surface in real time under colonoscopic observation. The imaging agent, which is named the nanobeacon, is fluorescent nanospheres conjugated with peanut agglutinin and poly(N-vinylacetamide). Its potential use as an imaging tool for colorectal cancer has been thoroughly validated in numerous studies. Here, toxicities of the nanobeacon were assessed in rats. The nanobeacon was prepared according to the synthetic manner which is being established as the Good Manufacturing Practice-guided production. The rat study was performed in accordance with Good Laboratory Practice regulations. No nanobeacon treatment-related toxicity was observed. The no observable adverse effect levels (NOAEL) of the nanobeacon in 7-day consecutive oral administration and single intrarectal administration were estimated to be more than 1000mg/kg/day and 50mg/kg/day, respectively. We concluded that the nanobeacon could be developed as a safe diagnostic agent for colonoscopy applications. FROM THE CLINICAL EDITOR:Colon cancer remains a major cause of death. Early detection can result in early treatment and thus survival. In this article, the authors tested potential systemic toxicity of coumarin 6-encapsulated polystyrene nanospheres conjugated with peanut agglutinin (PNA) and poly(N-vinylacetamide) (PNVA), which had been shown to bind specifically to colonic cancer cells and thus very promising in colonoscopic detection of cancer cells. |
| 巻・号 | 11(5) |
| ページ | 1227-36 |
| 公開日 | 2015-7-1 |
| DOI | 10.1016/j.nano.2015.02.003 |
| PII | S1549-9634(15)00049-0 |
| PMID | 25725490 |
| PMC | PMC4476914 |
| MeSH | Acetamides / administration & dosage Acetamides / chemistry Acetamides / toxicity* Animals Body Weight / drug effects CHO Cells Caco-2 Cells Colon / drug effects Colon / pathology Colonoscopy* Colorectal Neoplasms / diagnosis Coumarins / administration & dosage Coumarins / chemistry Coumarins / toxicity* Cricetulus Drinking / drug effects Eating / drug effects Fluorescent Dyes / administration & dosage Fluorescent Dyes / chemistry Fluorescent Dyes / toxicity* Humans Male Nanospheres / administration & dosage Nanospheres / chemistry Nanospheres / toxicity* Peanut Agglutinin / administration & dosage Peanut Agglutinin / chemistry Peanut Agglutinin / toxicity* Polystyrenes / administration & dosage Polystyrenes / chemistry Polystyrenes / toxicity* Polyvinyls / administration & dosage Polyvinyls / chemistry Polyvinyls / toxicity* Rats Rectum / drug effects Rectum / pathology Thiazoles / administration & dosage Thiazoles / chemistry Thiazoles / toxicity* |
| IF | 4.3 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) CHO |