論文 - 詳細
| RRC ID | 62102 |
|---|---|
| 著者 | Seta H, Matsuura K, Sekine H, Yamazaki K, Shimizu T. |
| タイトル | Tubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo. |
| ジャーナル | Sci Rep |
| Abstract |
Human induced pluripotent stem (iPS) cell-derived cardiac cells provide the possibility to fabricate cardiac tissues for transplantation. However, it remains unclear human bioengineered cardiac tissues function as a functional pump in vivo. Human iPS cells induced to cardiomyocytes in suspension were cultured on temperature-responsive dishes to fabricate cardiac cell sheets. Two pairs of triple-layered sheets were transplanted to wrap around the inferior vena cava (IVC) of nude rats. At 4 weeks after transplantation, inner pressure changes in the IVC were synchronized with electrical activations of the graft. Under 80 pulses per minute electrical stimulation, the inner pressure changes at 8 weeks increased to 9.1 ± 3.2 mmHg, which were accompanied by increases in the baseline inner pressure of the IVC. Immunohistochemical analysis revealed that 0.5-mm-thick cardiac troponin T-positive cardiac tissues, which contained abundant human mitochondria, were clearly engrafted lamellar around the IVC and surrounded by von Willebrand factor-positive capillary vessels. The mRNA expression of several contractile proteins in cardiac tissues at 8 weeks in vivo was significantly upregulated compared with those at 4 weeks. We succeeded in generating pulse pressure by tubular human cardiac tissues in vivo. This technology might lead to the development of a bioengineered heart assist pump. |
| 巻・号 | 7 |
| ページ | 45499 |
| 公開日 | 2017-3-30 |
| DOI | 10.1038/srep45499 |
| PII | srep45499 |
| PMID | 28358136 |
| PMC | PMC5371992 |
| MeSH | Animals Cell Differentiation Electric Stimulation Heart / physiology Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Mitochondria / metabolism Mitochondria / pathology Myocytes, Cardiac / cytology Myocytes, Cardiac / metabolism Myocytes, Cardiac / transplantation* Myosin Light Chains / genetics Myosin Light Chains / metabolism Rats Rats, Nude Tissue Engineering Tissue Scaffolds Troponin T / metabolism Vena Cava, Inferior / physiology* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |