| RRC ID |
62102
|
| 著者 |
Seta H, Matsuura K, Sekine H, Yamazaki K, Shimizu T.
|
| タイトル |
Tubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Human induced pluripotent stem (iPS) cell-derived cardiac cells provide the possibility to fabricate cardiac tissues for transplantation. However, it remains unclear human bioengineered cardiac tissues function as a functional pump in vivo. Human iPS cells induced to cardiomyocytes in suspension were cultured on temperature-responsive dishes to fabricate cardiac cell sheets. Two pairs of triple-layered sheets were transplanted to wrap around the inferior vena cava (IVC) of nude rats. At 4 weeks after transplantation, inner pressure changes in the IVC were synchronized with electrical activations of the graft. Under 80 pulses per minute electrical stimulation, the inner pressure changes at 8 weeks increased to 9.1 ± 3.2 mmHg, which were accompanied by increases in the baseline inner pressure of the IVC. Immunohistochemical analysis revealed that 0.5-mm-thick cardiac troponin T-positive cardiac tissues, which contained abundant human mitochondria, were clearly engrafted lamellar around the IVC and surrounded by von Willebrand factor-positive capillary vessels. The mRNA expression of several contractile proteins in cardiac tissues at 8 weeks in vivo was significantly upregulated compared with those at 4 weeks. We succeeded in generating pulse pressure by tubular human cardiac tissues in vivo. This technology might lead to the development of a bioengineered heart assist pump.
|
| 巻・号 |
7
|
| ページ |
45499
|
| 公開日 |
2017-3-30
|
| DOI |
10.1038/srep45499
|
| PII |
srep45499
|
| PMID |
28358136
|
| PMC |
PMC5371992
|
| MeSH |
Animals
Cell Differentiation
Electric Stimulation
Heart / physiology
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Mitochondria / metabolism
Mitochondria / pathology
Myocytes, Cardiac / cytology
Myocytes, Cardiac / metabolism
Myocytes, Cardiac / transplantation*
Myosin Light Chains / genetics
Myosin Light Chains / metabolism
Rats
Rats, Nude
Tissue Engineering
Tissue Scaffolds
Troponin T / metabolism
Vena Cava, Inferior / physiology*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
