| RRC ID |
62133
|
| 著者 |
Sasaki M, Sasaki T, Enami J.
|
| タイトル |
Prolactin-dependent growth and gamma-casein gene expression in Ba/F3 cells transfected with a long form of mouse mammary prolactin receptor.
|
| ジャーナル |
Endocr J
|
| Abstract |
Complementary DNA (cDNA) encoding a long form of prolactin receptor (PRL-RL) was cloned from mouse mammary gland by PCR using primers designed from the noncoding regions of previously reported rat ovarian PRL-RL cDNA. The nucleotide sequence encoding the extracellular and transmembrane domains of PRL-RL is completely identical to that of short forms of mouse PRL-R. The amino acid sequence deduced from the nucleotide sequence of mouse PRL-RL is 91% identical to that of rat PRL-RL. To address the question of whether or not the cloned mouse PRL-RL cDNA encodes a functional PRL-RL we transfected Ba/F3 IL-3-dependent murine pro-B lymphoid cells with the cDNA. By culturing the transfected cells in a medium which contained PRL in place of IL-3, we selected 5 PRL-dependent clones. All of these PRL-dependent clones, BaF/PD cells, expressed PRL-RL mRNA. In addition, BaF/PD cells expressed mammary-specific gamma-casein mRNA in response to PRL and dexamethasone. Based on these results, it was concluded that the mouse mammary PRL-RL cDNA cloned in this study is functionally active in mediating both PRL-dependent growth and mammary-specific gene expression.
|
| 巻・号 |
43(1)
|
| ページ |
45-52
|
| 公開日 |
1996-2-1
|
| DOI |
10.1507/endocrj.43.45
|
| PMID |
8732451
|
| MeSH |
Amino Acid Sequence
Animals
B-Lymphocytes / metabolism
Base Sequence
Caseins / genetics*
Cell Division / drug effects
Cell Line
Cloning, Molecular
DNA, Complementary / chemistry
DNA, Complementary / genetics
Female
Gene Expression*
Mammary Glands, Animal / chemistry*
Mice
Molecular Sequence Data
Prolactin / pharmacology*
RNA, Messenger / metabolism
Rats
Receptors, Prolactin / chemistry
Receptors, Prolactin / genetics*
Transfection*
|
| IF |
1.952
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805)
WEHI-3(RCB0035) |
