| RRC ID |
62138
|
| 著者 |
Kimura K, Asami K, Yamamoto M.
|
| タイトル |
Effect of heat shock treatment on the production of variant testosterone-repressed prostate message-2 (TRPM-2) mRNA in culture cells.
|
| ジャーナル |
Cell Biochem Funct
|
| Abstract |
The testosterone-repressive prostate message-2 (TRPM-2) variant mRNA lacking the exon 5 was induced in rat primary culture hepatocytes by heat shock treatment. A similar variant mRNA lacking exon 5 was also induced by heat shock treatment of the human culture cell line HepG2. On the other hand, in mouse cell line L929, heat shock treatment induced a variant TRPM-2 mRNA lacking only a small region located in exon 5. However, irrespective of the difference of mechanism of variant production, all the variant TRPM-2 mRNA species derived from each animal species encoded a putative protein constituted from the N-terminal one-third of TRPM-2 protein attached to a C-terminal TRPM-2 unrelated tail. In humans, the variant TRPM-2 species was not detected in normal tissues but was present in certain kinds of tumour cells. These results indicate that the splicing variants were induced as a direct result of heat shock treatment on cells per se and that the phenomenon of heat shock induction was observed in culture cells derived from different animal species.
|
| 巻・号 |
15(4)
|
| ページ |
251-7
|
| 公開日 |
1997-12-1
|
| DOI |
10.1002/(SICI)1099-0844(199712)15:4<251::AID-CBF748>3.0.CO;2-3
|
| PII |
10.1002/(SICI)1099-0844(199712)15:4<251::AID-CBF748>3.0.CO;2-3
|
| PMID |
9415971
|
| MeSH |
Alternative Splicing / physiology
Animals
Carcinoma, Hepatocellular
Clusterin
Complement Inactivator Proteins / genetics*
Cycloheximide / pharmacology
Fibroblasts / cytology
Fibroblasts / physiology
Gene Expression Regulation, Neoplastic / drug effects
Gene Expression Regulation, Neoplastic / genetics
Glycoproteins / genetics*
Heat-Shock Response / physiology*
Humans
Liver / cytology
Mice
Molecular Chaperones*
Neoplasm Proteins / genetics
Protein Synthesis Inhibitors / pharmacology
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Tumor Cells, Cultured
|
| IF |
2.632
|
| リソース情報 |
| ヒト・動物細胞 |
L929(RCB0081)
Hep G2(RCB0459) |
