論文 - 詳細
| RRC ID | 62142 |
|---|---|
| 著者 | Nabeshima K, Shimao Y, Inoue T, Itoh H, Kataoka H, Koono M. |
| タイトル | Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells. |
| ジャーナル | Int J Cancer |
| Abstract |
We presented earlier a 2-dimensional cell-motility assay using a highly metastatic variant (L-10) of human rectal-adenocarcinoma cell line RCM-1 as a motility model of tumor cells of epithelial origin. In this model, L-10 cells moved as coherent cell sheets when stimulated with 12-O-tetradecanoylphorbol-13-acetate (TPA), and we called this type of movement "cohort migration". Electron- and immunoelectron-microscope study of the migrating cell sheets demonstrated localized release from cell-cell adhesion only at the lower portion of the cells with loss of E-cadherin immunoreactivity, and this change was associated with increased tyrosine phosphorylation of the E-cadherin-catenin complex, including beta-catenin. In the present study, to obtain evidence to support the relevance of our model to carcinoma-cell movement in vivo, we sought a naturally occurring motogenic factor(s) able to induce this cohort migration. Among the factors examined, hepatocyte growth factor/scatter factor (HGF/SF) clearly induced cohort migration of L-10 cells. Additionally, not only L-10 but several other human colorectal-carcinoma cell lines showed this type of migration in response to HGF/SF, while yet others showed scattering-type motility. In this HGF/SF-induced migration, localized release from cell-cell adhesion was induced only at the lower portion of the cells, allowing them to extend leading lamellae, whereas close cell-cell contacts remained at the upper portion of the cells, as seen in TPA-induced cohort migration. Scattering-type cell lines tended to express more c-Met (receptor for HGF/SF) mRNA than the cell lines that showed cohort-type migration. LoVo, one of the scattering-type cell lines, expressed more c-Met protein and less E-cadherin than L-10, which showed cohort-type migration. HGF/SF treatment of LoVo reduced the amount of alpha-catenin complexed with E-cadherin more markedly than in L-10, but in both cell lines this reduction was not accompanied by increased tyrosine phosphorylation of beta-catenin, suggesting the presence of a mechanism other than phosphorylation for release from cell-cell adhesion during cell motility. |
| 巻・号 | 78(6) |
| ページ | 750-9 |
| 公開日 | 1998-12-9 |
| DOI | 10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-# |
| PII | 10.1002/(SICI)1097-0215(19981209)78:6<750::AID-IJC13>3.0.CO;2-# |
| PMID | 9833769 |
| MeSH | Adenocarcinoma / pathology* Cadherins / metabolism Cell Adhesion / drug effects Cell Division / drug effects Cell Movement / drug effects Colorectal Neoplasms / pathology* Cytoskeletal Proteins / metabolism Hepatocyte Growth Factor / pharmacology* Humans Phosphorylation Recombinant Proteins / pharmacology Tumor Cells, Cultured alpha Catenin |
| IF | 5.145 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CW-2(RCB0778) |