RRC ID 62153
著者 Onodera S, Suzuki K, Kaneda K, Fujinaga M, Nishihira J.
タイトル Growth factor-induced expression of macrophage migration inhibitory factor in osteoblasts: relevance to the plasminogen activator system.
ジャーナル Semin Thromb Hemost
Abstract Macrophage migration inhibitory factor (MIF) is a cytokine that mediates inflammatory processes in a variety of tissues. In this study, we examined the expression of MIF mRNA in the mouse osteoblastic cell line MC3T3-E1, whose proliferation is promoted by various growth factors. In the subconfluent state, transforming growth factor-beta, basic fibroblast growth factor, insulin-like growth factor-II, and fetal calf serum markedly upregulated MIF mRNA expression. The upregulation of MIF mRNA was less extensive when the cells were stimulated by the same growth factors in the overconfluent state. We also investigated the expression of MIF mRNA through a whole cell cycle from G0 phase when the osteoblastic cells were synchronized by serum starvation. The MIF mRNA expression, which gradually increased from the G0 and reached its maximum at the S phase, was nonperiodic. Moreover, human recombinant MIF upregulated the expression of urokinase plasminogen activator inhibitor-1 (PAI-1) precursor mRNA in human osteoblastic Saos-2 cells. Plasminogen activator (PA) is known to play an important role in bone metabolism, for example, in activation of procollagenase or growth factors indirectly via the formation of plasmin, and in mitogenic activity for osteoblastic cells. Our results suggest that MIF modulates the proliferation of osteoblasts and, moreover, bone tissue remodeling through the PA and plasmin system.
巻・号 25(6)
ページ 563-8
公開日 1999-1-1
DOI 10.1055/s-2007-994966
PMID 10632479
MeSH Animals Bone Remodeling / drug effects Cell Cycle / genetics Cell Line / chemistry Cell Line / metabolism Growth Substances / pharmacology Humans Macrophage Migration-Inhibitory Factors / biosynthesis Macrophage Migration-Inhibitory Factors / genetics Macrophage Migration-Inhibitory Factors / pharmacology Mice Osteoblasts / chemistry* Plasminogen Activator Inhibitor 1 / biosynthesis Plasminogen Activator Inhibitor 1 / genetics Plasminogen Activators / physiology Protein Precursors / biosynthesis Protein Precursors / genetics RNA, Messenger / biosynthesis RNA, Messenger / drug effects
IF 2.892
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)