論文 - 詳細
| RRC ID | 62158 |
|---|---|
| 著者 | Muramatsu M, Hanazono Y, Ogasawara Y, Okada T, Mizukami H, Kume A, Mizoguchi H, Ozawa K. |
| タイトル | Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Since hematopoietic stem cells (HSCs) differentiate readily ex vivo resulting in the loss of self-renewal and engraftment abilities, the transient block of differentiation is essential to maintain those abilities during their ex vivo expansion culture. To this end, we developed a method of reversible integration of the dominant negative retinoic acid receptor (DN-RAR) gene, a differentiation-blocking gene, into cells utilizing the Cre/loxP-dependent gene recombination system. The murine immature hematopoietic 32D cells differentiate into mature neutrophils upon G-CSF treatment. However, 32D cells transduced with a retroviral vector expressing the DN-RAR gene put between two loxP sites continued to proliferate without showing differentiation even in the presence of G-CSF. After the cells were fully amplified, the cells were transduced with the Cre recombinase gene. The cells then restored the ability to differentiate into mature neutrophils upon G-CSF treatment. PCR analysis showed that the DN-RAR gene was efficiently removed from the genome by introduction of the Cre gene. This system may eventually be applicable to the ex vivo expansion of HSCs. |
| 巻・号 | 285(4) |
| ページ | 891-6 |
| 公開日 | 2001-7-27 |
| DOI | 10.1006/bbrc.2001.5264 |
| PII | S0006-291X(01)95264-7 |
| PMID | 11467834 |
| MeSH | Animals Cell Culture Techniques / methods Cell Differentiation Cell Division Hematopoietic Stem Cells / cytology* Integrases Mice Neutrophils / cytology* Receptors, Retinoic Acid / genetics* Recombination, Genetic Transformation, Genetic Viral Proteins |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |