論文 - 詳細
| RRC ID | 62169 |
|---|---|
| 著者 | Becker JC, Hertel M, Markmann A, Shahin M, Werner S, Domschke W, Pohle T. |
| タイトル | Dynamics and localization of activin A expression in rat gastric ulcers. |
| ジャーナル | Scand J Gastroenterol |
| Abstract |
BACKGROUND:Activin A, the homodimer of the activin/inhibin betaA subunit, has been shown to participate in cutaneous wound healing. In this study we intended to determine its part in gastric ulceration. METHODS:Activin A expression was studied by immunohistochemistry and in situ hybridization in acetic-acid-induced chronic gastric ulcers in rat. The dynamics of this process were also assessed by quantitative real time RT-PCR and RNase protection assays (RPA). The effects of different doses of this cytokine on epithelial and mesenchymal cell proliferation were quantitated in vitro. RESULTS:Low amounts of activin A and its mRNA were expressed by epithelia, endothelia and fibroblasts in intact gastric tissue. Granulation tissue of gastric ulcers and gastric glands adjacent to the ulcer rim expressed markedly increased amounts of activin protein as well as activin/inhibin betaA mRNA. RPA and RT-PCR studies revealed a more than 3-fold increase in the relative abundance of this mRNA. Activin A did not affect the proliferation rate of fibroblasts and epithelial cells in vitro. CONCLUSIONS:Activin A participates in gastric ulcer healing in a similar fashion as in cutaneous wounding. Its expression on protein and mRNA level is markedly increased in ulcer base and rim. |
| 巻・号 | 38(3) |
| ページ | 260-7 |
| 公開日 | 2003-3-1 |
| DOI | 10.1080/00365520310000636a |
| PMID | 12737440 |
| MeSH | Activins / administration & dosage Activins / biosynthesis* Activins / genetics* Animals Biomarkers / analysis Cell Count Cell Division / drug effects Cell Division / physiology Disease Models, Animal Dose-Response Relationship, Drug Epithelial Cells / cytology Epithelial Cells / metabolism Extracellular Matrix / metabolism Fibroblasts / cytology Gastric Mucosa / cytology Gastric Mucosa / metabolism Gene Expression Immunohistochemistry In Situ Hybridization Inhibin-beta Subunits / administration & dosage Inhibin-beta Subunits / biosynthesis* Inhibin-beta Subunits / genetics* Male Mucous Membrane / cytology Mucous Membrane / metabolism RNA, Messenger / metabolism Rats Rats, Wistar Stomach Ulcer / genetics* Stomach Ulcer / metabolism* Time Factors |
| IF | 2.13 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |