| RRC ID |
62170
|
| 著者 |
Kumagai H, Oki T, Tamitsu K, Feng SZ, Ono M, Nakajima H, Bao YC, Kawakami Y, Nagayoshi K, Copeland NG, Gilbert DJ, Jenkins NA, Kawakami T, Kitamura T.
|
| タイトル |
Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We have identified and characterized two mouse cDNAs in a mouse antigen-stimulated bone marrow-derived mast cell cDNA library, both of which encode type I transmembrane proteins. The genes were closely mapped in the distal region of mouse chromosome 11 and expressed not only in mast cells but also widely in leukocytes. The extracellular domains of their encoded proteins contain a single variable immunoglobulin (Ig) motif sharing about 90% identity with amino acids, showing that they comprise a pair of molecules and belong to the Ig superfamily. We named these molecules leukocyte mono-Ig-like receptor1 and 2 (LMIR1 and 2). The intracellular domain of LMIR1 contains several immunoreceptor tyrosine-based inhibition motifs (ITIMs). When cross-linked, the intracellular domain was tyrosine phosphorylated and capable of recruiting tyrosine phosphatases, SHP-1 and SHP-2 and inositol polyphosphate 5-phosphatase, SHIP. LMIR2, on the other hand, contains a short cytoplasmic tail and a characteristic transmembrane domain carrying two positively charged amino acids associated with three kinds of immunoreceptor tyrosine-based activation motif (ITAM)-bearing molecules, DAP10, DAP12, and FcRgamma. These findings suggest that a new pair of ITIM/ITAM-bearing receptors, LMIR1 and 2, regulate mast cell-mediated inflammatory responses through yet to be defined ligand(s).
|
| 巻・号 |
307(3)
|
| ページ |
719-29
|
| 公開日 |
2003-8-1
|
| DOI |
10.1016/s0006-291x(03)01245-2
|
| PII |
S0006291X03012452
|
| PMID |
12893283
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chromosome Mapping
Cloning, Molecular
Hematopoietic Stem Cells / physiology
Mast Cells / immunology*
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
Phosphoric Monoester Hydrolases / metabolism
Phosphorylation
Protein Structure, Tertiary
Protein Tyrosine Phosphatases / metabolism
RNA, Messenger / biosynthesis
Receptors, Immunologic / chemistry
Receptors, Immunologic / genetics*
Receptors, Immunologic / metabolism*
Tyrosine / metabolism
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
32Dcl3(RCB1538) |
