RRC ID 62175
著者 Gabison EE, Mourah S, Steinfels E, Yan L, Hoang-Xuan T, Watsky MA, De Wever B, Calvo F, Mauviel A, Menashi S.
タイトル Differential expression of extracellular matrix metalloproteinase inducer (CD147) in normal and ulcerated corneas: role in epithelio-stromal interactions and matrix metalloproteinase induction.
ジャーナル Am J Pathol
Abstract Extracellular matrix metalloproteinase inducer (EMMPRIN) was originally identified on the tumor cell surface as an inducer of matrix metalloproteinase (MMP) production in neighboring fibroblasts. Here we demonstrate a role for EMMPRIN in MMP induction during corneal wound healing. MMP and EMMPRIN expression was analyzed in normal and ulcerated human corneas, as well as in corneal epithelial and stromal cells in culture using confocal microscopy, zymography, immunoblots, and real-time polymerase chain reaction. In normal cornea EMMPRIN was predominantly expressed in the epithelium but was markedly induced in the anterior stroma of ulcerated corneas. This coincided with MMP-2 induction that co-localized with EMMPRIN at the epithelio-stromal boundary. The role of epithelial-stromal interaction in MMP induction was investigated in an in vitro co-culture system and demonstrated an induction and co-localization of EMMPRIN and MMP-2 in the fibroblasts at the interface with epithelial cells. Direct contact of fibroblasts with EMMPRIN-containing purified epithelial cell membranes also induced MMP-1, MMP-2, and EMMPRIN and this was inhibited by a blocking anti-EMMPRIN antibody, suggesting that EMMPRIN was primarily responsible for this induction. These findings, and the up-regulation of EMMPRIN by epidermal growth factor and transforming growth factor-beta, demonstrate a role for EMMPRIN in wound healing and suggest that sustained local up-regulation of EMMPRIN and MMPs in chronic situations in which healing is delayed may lead to excessive matrix degradation and corneal melts.
巻・号 166(1)
ページ 209-19
公開日 2005-1-1
DOI 10.1016/S0002-9440(10)62245-6
PII S0002-9440(10)62245-6
PMID 15632013
PMC PMC1602288
MeSH Antigens, CD / genetics* Antigens, Neoplasm / genetics* Basigin Cell Line Cholera Toxin / pharmacology Coculture Techniques Cornea / cytology Cornea / immunology* Corneal Ulcer / immunology* Corneal Ulcer / pathology DNA Primers Epidermal Growth Factor / pharmacology Epithelium, Corneal / cytology Epithelium, Corneal / immunology Epithelium, Corneal / pathology Fibroblasts / cytology Fibroblasts / drug effects Fibroblasts / immunology Humans Immunohistochemistry Insulin / pharmacology Microscopy, Confocal Polymerase Chain Reaction Reference Values Transforming Growth Factor beta / pharmacology Transforming Growth Factor beta1
IF 3.491
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)