RRC ID |
62175
|
著者 |
Gabison EE, Mourah S, Steinfels E, Yan L, Hoang-Xuan T, Watsky MA, De Wever B, Calvo F, Mauviel A, Menashi S.
|
タイトル |
Differential expression of extracellular matrix metalloproteinase inducer (CD147) in normal and ulcerated corneas: role in epithelio-stromal interactions and matrix metalloproteinase induction.
|
ジャーナル |
Am J Pathol
|
Abstract |
Extracellular matrix metalloproteinase inducer (EMMPRIN) was originally identified on the tumor cell surface as an inducer of matrix metalloproteinase (MMP) production in neighboring fibroblasts. Here we demonstrate a role for EMMPRIN in MMP induction during corneal wound healing. MMP and EMMPRIN expression was analyzed in normal and ulcerated human corneas, as well as in corneal epithelial and stromal cells in culture using confocal microscopy, zymography, immunoblots, and real-time polymerase chain reaction. In normal cornea EMMPRIN was predominantly expressed in the epithelium but was markedly induced in the anterior stroma of ulcerated corneas. This coincided with MMP-2 induction that co-localized with EMMPRIN at the epithelio-stromal boundary. The role of epithelial-stromal interaction in MMP induction was investigated in an in vitro co-culture system and demonstrated an induction and co-localization of EMMPRIN and MMP-2 in the fibroblasts at the interface with epithelial cells. Direct contact of fibroblasts with EMMPRIN-containing purified epithelial cell membranes also induced MMP-1, MMP-2, and EMMPRIN and this was inhibited by a blocking anti-EMMPRIN antibody, suggesting that EMMPRIN was primarily responsible for this induction. These findings, and the up-regulation of EMMPRIN by epidermal growth factor and transforming growth factor-beta, demonstrate a role for EMMPRIN in wound healing and suggest that sustained local up-regulation of EMMPRIN and MMPs in chronic situations in which healing is delayed may lead to excessive matrix degradation and corneal melts.
|
巻・号 |
166(1)
|
ページ |
209-19
|
公開日 |
2005-1-1
|
DOI |
10.1016/S0002-9440(10)62245-6
|
PII |
S0002-9440(10)62245-6
|
PMID |
15632013
|
PMC |
PMC1602288
|
MeSH |
Antigens, CD / genetics*
Antigens, Neoplasm / genetics*
Basigin
Cell Line
Cholera Toxin / pharmacology
Coculture Techniques
Cornea / cytology
Cornea / immunology*
Corneal Ulcer / immunology*
Corneal Ulcer / pathology
DNA Primers
Epidermal Growth Factor / pharmacology
Epithelium, Corneal / cytology
Epithelium, Corneal / immunology
Epithelium, Corneal / pathology
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / immunology
Humans
Immunohistochemistry
Insulin / pharmacology
Microscopy, Confocal
Polymerase Chain Reaction
Reference Values
Transforming Growth Factor beta / pharmacology
Transforming Growth Factor beta1
|
IF |
3.491
|
リソース情報 |
ヒト・動物細胞 |
HCE-T(RCB2280) |