| RRC ID |
62177
|
| 著者 |
Ohashi N, Kodera Y, Nakanishi H, Yokoyama H, Fujiwara M, Koike M, Hibi K, Nakao A, Tatematsu M.
|
| タイトル |
Efficacy of intraperitoneal chemotherapy with paclitaxel targeting peritoneal micrometastasis as revealed by GFP-tagged human gastric cancer cell lines in nude mice.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
To explore the effect of chemotherapy against peritoneal micrometastases, we used in vivo models of peritoneal micrometastasis featuring three human gastric cancer cell lines tagged with the green fluorescence protein gene. With a highly metastatic cell line, GCIY-EGFP, the survival rate of mice treated with weekly intraperitoneal paclitaxel starting one day following inoculation was significantly higher than those of control mice or mice given the same treatment starting on day eight. Intravenous paclitaxel exhibited no significant survival benefit regardless of the treatment schedule. Pharmacokinetic analysis revealed that the concentration of paclitaxel in the metastatic nodules treated with intraperitoneal administration was 2-3-fold higher than those treated with intravenous paclitaxel, provided the treatment was given on day one. Even greater responses were observed with less aggressive MKN28-EGFP and MKN45-EGFP cell lines, whose peritoneal metastases resolved completely due to early intraperitoneal treatment in 13 of the 14 mice tested. These results indicate that intraperitoneal paclitaxel is effective against peritoneal metastasis in early phase.
|
| 巻・号 |
27(3)
|
| ページ |
637-44
|
| 公開日 |
2005-9-1
|
| PMID |
16077911
|
| MeSH |
Animals
Antineoplastic Agents, Phytogenic / administration & dosage*
Antineoplastic Agents, Phytogenic / pharmacokinetics
Antineoplastic Agents, Phytogenic / therapeutic use
Cell Line, Tumor
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Immunohistochemistry
Injections, Intraperitoneal
Injections, Intravenous
Male
Mice
Mice, Nude
Neovascularization, Pathologic / metabolism
Neovascularization, Pathologic / pathology
Paclitaxel / administration & dosage*
Paclitaxel / pharmacokinetics
Paclitaxel / therapeutic use
Peritoneal Neoplasms / drug therapy*
Peritoneal Neoplasms / mortality
Peritoneal Neoplasms / secondary
Plasmids / genetics
Platelet Endothelial Cell Adhesion Molecule-1 / analysis
Stomach Neoplasms / drug therapy*
Stomach Neoplasms / genetics
Stomach Neoplasms / pathology
Survival Analysis
Survival Rate
Transfection
Treatment Outcome
Xenograft Model Antitumor Assays / methods
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001) |
