論文 - 詳細
| RRC ID | 62185 |
|---|---|
| 著者 | Awong G, La Motte-Mohs RN, Zúñiga-Pflücker JC. |
| タイトル | In vitro human T cell development directed by notch-ligand interactions. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Traditionally, the study of human T cell development has relied on the availability of human and mouse thymic tissue. In this chapter, we outline a simple in vitro protocol for generating large numbers of human T-lineage cells from umbilical cord blood (CB)- derived hematopoietic stem cells (HSCs) using a bone marrow stromal cell line. This protocol is broken into three major steps: (1) the maintenance of a working stock of OP9 bone marrow stromal cells expressing the Notch receptor ligand Delta-like 1 (OP9- DL1), (2) the purification of human HSCs from umbilical CB, and (3) the initiation and maintenance/expansion of OP9-DL1 cocultures over time (see Fig. 1). The use of this system opens avenues for basic research as it equips us with a simple in vitro method for studying human T cell development. |
| 巻・号 | 430 |
| ページ | 135-42 |
| 公開日 | 2008-1-1 |
| DOI | 10.1007/978-1-59745-182-6_9 |
| PMID | 18370296 |
| MeSH | Cell Line Coculture Techniques Flow Cytometry Hematopoietic Stem Cells / cytology Humans Ligands Receptors, Notch / metabolism* T-Lymphocytes / cytology* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |