RRC ID 622
著者 Kuroda H, Kojima H, Kaneda H, Takashio M.
タイトル Characterization of 9-fatty acid hydroperoxide lyase-like activity in germinating barley seeds that transforms 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid into 2(E)-nonenal.
ジャーナル Biosci Biotechnol Biochem
Abstract Previously, we reported that 2(E)-nonenal, having a low flavor threshold (0.1 ppb) and known as the major contributor to a cardboard flavor (stale flavor) in stored beer, is produced by lipoxygenase-1 and a newly found factor named 9-fatty acid hydroperoxide lyase-like (9-HPL-like) activity in malt. To assess the involvement of 9-HPL-like activity in beer staling, we compared the values of the wort nonenal potential, an index for predicting the staleness of beer, with the lipoxygenase and 9-HPL-like activity of 20 commercial malts. There was a significant correlation between the malt 9-HPL-like activity and the values of wort nonenal potential (r=0.53, P<0.05), while the correlation between malt lipoxygenase activity and the wort nonenal potential was statistically insignificant. Analysis of the partially purified 9-HPL-like activity from embryos of germinating barley seeds indicated that 9-HPL-like activity consisted of fatty acid hydroperoxide lyase and 3Z:2E isomerase.
巻・号 69(9)
ページ 1661-8
公開日 2005-9-1
DOI 10.1271/bbb.69.1661
PII JST.JSTAGE/bbb/69.1661
PMID 16195582
MeSH Aldehyde-Lyases / chemistry* Aldehyde-Lyases / metabolism Aldehydes / chemistry* Aldehydes / metabolism Cytochrome P-450 Enzyme System / chemistry* Cytochrome P-450 Enzyme System / metabolism Gas Chromatography-Mass Spectrometry Germination Hordeum / enzymology* Isomerases / chemistry Isomerases / metabolism Linoleic Acids / chemistry* Linoleic Acids / metabolism Lipid Peroxides / chemistry* Lipid Peroxides / metabolism Seeds / enzymology*
IF 1.516
引用数 6
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
オオムギ Haruna Nijo?