論文 - 詳細
RRC ID | 622 |
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著者 | Kuroda H, Kojima H, Kaneda H, Takashio M. |
タイトル | Characterization of 9-fatty acid hydroperoxide lyase-like activity in germinating barley seeds that transforms 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid into 2(E)-nonenal. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Previously, we reported that 2(E)-nonenal, having a low flavor threshold (0.1 ppb) and known as the major contributor to a cardboard flavor (stale flavor) in stored beer, is produced by lipoxygenase-1 and a newly found factor named 9-fatty acid hydroperoxide lyase-like (9-HPL-like) activity in malt. To assess the involvement of 9-HPL-like activity in beer staling, we compared the values of the wort nonenal potential, an index for predicting the staleness of beer, with the lipoxygenase and 9-HPL-like activity of 20 commercial malts. There was a significant correlation between the malt 9-HPL-like activity and the values of wort nonenal potential (r=0.53, P<0.05), while the correlation between malt lipoxygenase activity and the wort nonenal potential was statistically insignificant. Analysis of the partially purified 9-HPL-like activity from embryos of germinating barley seeds indicated that 9-HPL-like activity consisted of fatty acid hydroperoxide lyase and 3Z:2E isomerase. |
巻・号 | 69(9) |
ページ | 1661-8 |
公開日 | 2005-9-1 |
DOI | 10.1271/bbb.69.1661 |
PII | JST.JSTAGE/bbb/69.1661 |
PMID | 16195582 |
MeSH | Aldehyde-Lyases / chemistry* Aldehyde-Lyases / metabolism Aldehydes / chemistry* Aldehydes / metabolism Cytochrome P-450 Enzyme System / chemistry* Cytochrome P-450 Enzyme System / metabolism Gas Chromatography-Mass Spectrometry Germination Hordeum / enzymology* Isomerases / chemistry Isomerases / metabolism Linoleic Acids / chemistry* Linoleic Acids / metabolism Lipid Peroxides / chemistry* Lipid Peroxides / metabolism Seeds / enzymology* |
IF | 1.516 |
引用数 | 6 |
WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
オオムギ | Haruna Nijo? |