RRC ID 62200
著者 Gümüşderelioğlu M, Aday S.
タイトル Heparin-functionalized chitosan scaffolds for bone tissue engineering.
ジャーナル Carbohydr Res
Abstract The aim of this study is to investigate the effects of heparin-functionalized chitosan scaffolds on the activity of preosteoblasts. The chitosan scaffolds having the pore size of ∼100 μm were prepared by a freeze-drying method. Two different methods for immobilization of heparin to chitosan scaffolds were successfully performed. In the first method, functionalization of the scaffolds was achieved by means of electrostatic interactions between negatively charged heparin and positively charged chitosan. The covalent immobilization of heparin to chitosan scaffolds by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC) and N-hydroxysuccinimide (NHS) was used as a second immobilization method. Morphology, proliferation, and differentiation of MC3T3-E1 preosteoblasts on heparin-functionalized chitosan scaffolds were investigated in vitro. The results indicate that covalently bound heparin containing chitosan scaffolds (CHC) stimulate osteoblast proliferation compared to other scaffolds, that is, unmodified chitosan scaffolds (CH), electrostatically bound heparin containing chitosan scaffolds (EHC), and CH+free heparin (CHF). SEM images also proved the stimulative effect of covalently bound heparin on the proliferation of preosteoblasts. Alkaline phosphatase (ALP) and osteocalcin (OCN) levels of cells proliferated on CHC and EHC were also higher than those for CH and CHF. In vitro studies have demonstrated that chitosan scaffolds increase viability and differentiation of MC3T3-E1 cells especially in the presence of immobilized heparin.
巻・号 346(5)
ページ 606-13
公開日 2011-4-1
DOI 10.1016/j.carres.2010.12.007
PII S0008-6215(10)00531-8
PMID 21333274
MeSH Animals Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Cell Differentiation / drug effects Cell Line Chitosan / chemistry* Heparin / chemistry* Mice Osteogenesis / drug effects Static Electricity Tissue Engineering / methods* Tissue Scaffolds / chemistry*
IF 1.841
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)