RRC ID 62203
著者 Igarashi M, Sakamoto K, Nagaoka I.
タイトル Effect of glucosamine, a therapeutic agent for osteoarthritis, on osteoblastic cell differentiation.
ジャーナル Int J Mol Med
Abstract Osteoarthritis (OA) is characterized by qualitative and quantitative changes in the architecture and composition of all the joint structures. Glucosamine (GlcN) has been used to treat OA in humans, because GlcN is present in the cartilage tissues as a component of glycosaminoglycans, and exhibits the symptom-modifying effect on OA by normalizing cartilage metabolism. On the other hand, the pathological change of subchondral bone is associated with the initiation and progression of cartilage damage in OA. However, the effect of GlcN on bone metabolism remains unsolved. In the present study, we determined the effect of GlcN on bone metabolism (osteoblastic cell differentiation) using mouse osteoblastic MC3T3-E1 cells by evaluating the expression of early (type I collagen and alkaline phosphatase), middle (osteopontin) and late (osteocalcin and mineralization) stage differentiation markers, and further compared its effects to those of N-acetyl-D-glucosamine (GlcNAc), a derivative of GlcN. The results indicated that the mineralization of mature osteoblasts was increased by treatment with GlcN and GlcNAc. Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that GlcN and GlcNAc substantially increased the expression of a middle stage marker and a late stage marker, although they did not essentially affect the expression of early stage markers. In addition, GlcN and GlcNAc suppressed the expression of receptor activator of NF-κB ligand (RANKL), a key factor involved in the osteoclastic cell differentiation and activation. Together these observations suggest that both GlcN and GlcNAc may have a potential not only to induce osteoblastic cell differentiation especially at middle-late stages, but also to suppress the osteoclastic cell differentiation, thereby possibly increasing bone matrix deposition and decreasing bone resorption, and eventually modulating bone metabolism in OA.
巻・号 28(3)
ページ 373-9
公開日 2011-9-1
DOI 10.3892/ijmm.2011.686
PMID 21537831
MeSH Acetylglucosamine / pharmacology Alkaline Phosphatase / metabolism Animals Cell Differentiation / drug effects* Cell Line Collagen Type I / metabolism Glucosamine / pharmacology* Glycosaminoglycans / metabolism Mice Osteoarthritis / drug therapy* Osteoarthritis / pathology Osteoblasts / cytology Osteoblasts / drug effects* Osteocalcin / metabolism Osteogenesis / drug effects Receptor Activator of Nuclear Factor-kappa B / antagonists & inhibitors Receptor Activator of Nuclear Factor-kappa B / drug effects Receptor Activator of Nuclear Factor-kappa B / genetics Reverse Transcriptase Polymerase Chain Reaction
IF 3.098
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)