RRC ID 62209
著者 Chen DS, Chan KM.
タイトル PCR-cloning of tilapia ATP7A cDNA and its mRNA levels in tissues of tilapia following copper administrations.
ジャーナル Aquat Toxicol
Abstract We are studying the toxicity of copper to tilapia and zebrafish and have found that the copper tolerance of tilapia and the sensitivity of zebrafish were due to several proteins' regulation mechanisms that were related to the effects of reactive oxygen species, mitochondrion copper transport, and stress response. To further reveal the mechanism of copper tolerance and sensitivity in tilapia and zebrafish, a full length cDNA of ATP7A was obtained in tilapia. Using real time quantitative PCR, the differential regulations of ATP7A in tilapia and zebrafish were studied. It was found that Cu(2+) gave a higher induction of ATP7A in tilapia than zebrafish, both in vivo and in vitro. These results suggest that the copper tolerance of tilapia may be due to higher expression level of ATP7A.
巻・号 105(3-4)
ページ 717-27
公開日 2011-10-1
DOI 10.1016/j.aquatox.2011.09.011
PII S0166-445X(11)00259-1
PMID 21996258
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Base Sequence Cloning, Molecular Copper / toxicity* Copper-Transporting ATPases DNA, Complementary / chemistry Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation / drug effects Molecular Sequence Data Phylogeny RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Tilapia / genetics Tilapia / metabolism* Water Pollutants, Chemical / toxicity* Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 4.346
リソース情報
ヒト・動物細胞 Hepa-T1(RCB1156)