RRC ID 62216
Author Stakleff KS, Lin F, Smith Callahan LA, Wade MB, Esterle A, Miller J, Graham M, Becker ML.
Title Resorbable, amino acid-based poly(ester urea)s crosslinked with osteogenic growth peptide with enhanced mechanical properties and bioactivity.
Journal Acta Biomater
Abstract Materials currently used for the treatment of bone defects include ceramics, polymeric scaffolds and composites, which are often impregnated with recombinant growth factors and other bioactive substances. While these materials have seen instances of success, each has inherent shortcomings including prohibitive expense, poor protein stability, poorly defined growth factor release and less than desirable mechanical properties. We have developed a novel class of amino acid-based poly(ester urea)s (PEU) materials which are biodegradable in vivo and possess mechanical properties superior to conventionally used polyesters (<3.5 GPa) available currently to clinicians and medical providers. We report the use of a short peptide derived from osteogenic growth peptide (OGP) as a covalent crosslinker for the PEU materials. In addition to imparting specific bioactive signaling, our crosslinking studies show that the mechanical properties increase proportionally when 0.5% and 1.0% concentrations of the OGP crosslinker are added. Our results in vitro and in an in vivo subcutaneous rat model show the OGP-based crosslinkers, which are small fragments of growth factors that are normally soluble, exhibit enhanced proliferative activity, accelerated degradation properties and concentration dependent bioactivity when immobilized.
Volume 9(2)
Pages 5132-42
Published 2013-2-1
DOI 10.1016/j.actbio.2012.08.035
PII S1742-7061(12)00411-4
PMID 22975625
MeSH Amino Acid Sequence Amino Acids / chemistry* Animals Biocompatible Materials / pharmacology* Cell Proliferation / drug effects Cross-Linking Reagents / chemistry* Elastic Modulus / drug effects Fibroblasts / cytology Fibroblasts / drug effects Histones / chemical synthesis Histones / chemistry Histones / pharmacology* Humans Intercellular Signaling Peptides and Proteins / chemical synthesis Intercellular Signaling Peptides and Proteins / chemistry Intercellular Signaling Peptides and Proteins / pharmacology* Male Materials Testing* Mechanical Phenomena / drug effects* Mice Molecular Sequence Data Polyesters / chemical synthesis Polyesters / chemistry* Rats Rats, Sprague-Dawley Tensile Strength / drug effects
IF 7.242
Human and Animal Cells MC3T3-E1(RCB1126)