論文 - 詳細
RRC ID | 62219 |
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著者 | Kato S, Saitoh Y, Miwa N. |
タイトル | Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes. |
ジャーナル | Int J Hyperthermia |
Abstract |
PURPOSE:The aim of this study was to evaluate inhibitory effects of L-ascorbic acid-2-O-phosphate-Na(2) (APS), a pro-vitamin C, combined with hyperthermia on adipogenic differentiation of mouse stromal cells, OP9. MATERIALS AND METHODS:OP9 preadipocytes were differentiated with serum replacement, administered with APS, and simultaneously treated with hyperthermia using a capacitive-resistive electric transfer (CRet) apparatus, which was conducted repeatedly twice a day. After 2 days, intracellular lipid droplets were stained with Oil Red O, then observed by microscopy and assessed spectrophotometrically. RESULTS:After stimulation by serum replacement for 2 days, lipid droplets were accumulated surrounding nucleus of OP9 cells. When APS of 0.15-0.6 mM was administered without hyperthermia, the amount of lipid droplets was markedly suppressed to 50.5%∼-11.3% versus the undifferentiated control, and diminished huge aggregates of lipid droplets. In OP9 cells treated by hyperthermia at 42°C for 0.5 min, 1 min or 3 min in the absence of APS, adipogenesis was suppressed abruptly in a time-dependent manner to 95.4%, 18.7% or -5.5%, respectively. Whereas, the percentage of adipogenesis was 96.8% in OP9 cells treated by mild hyperthermia alone at 41°C for 1 min. The simultaneous application of APS and hyperthermia at 41°C for 1 min markedly suppressed the accumulation of lipid droplets to 25.7%∼-66.2%. By scanning electron microscopy (SEM) observation, the surface of OP9 cells treated with APS and hyperthermia appeared to have the morphological property of undifferentiated OP9 cells. CONCLUSION:Combined treatment of APS and mild hyperthermia suppresses adipogenesis in OP9 cells, particularly in lipid droplets accumulation during spontaneous differentiation of OP9 preadipocytes. |
巻・号 | 29(1) |
ページ | 30-7 |
公開日 | 2013-1-1 |
DOI | 10.3109/02656736.2012.750016 |
PMID | 23286696 |
MeSH | Adipocytes / cytology Adipocytes / drug effects Adipocytes / metabolism Adipogenesis / drug effects* Animals Antineoplastic Agents / administration & dosage Antioxidants / administration & dosage* Ascorbic Acid / administration & dosage Ascorbic Acid / analogs & derivatives* Cell Line Hyperthermia, Induced* Lipid Metabolism / drug effects Mice Stromal Cells |
IF | 3.574 |
リソース情報 | |
ヒト・動物細胞 | OP9(RCB1124) |