RRC ID 62219
著者 Kato S, Saitoh Y, Miwa N.
タイトル Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes.
ジャーナル Int J Hyperthermia
Abstract PURPOSE:The aim of this study was to evaluate inhibitory effects of L-ascorbic acid-2-O-phosphate-Na(2) (APS), a pro-vitamin C, combined with hyperthermia on adipogenic differentiation of mouse stromal cells, OP9.
MATERIALS AND METHODS:OP9 preadipocytes were differentiated with serum replacement, administered with APS, and simultaneously treated with hyperthermia using a capacitive-resistive electric transfer (CRet) apparatus, which was conducted repeatedly twice a day. After 2 days, intracellular lipid droplets were stained with Oil Red O, then observed by microscopy and assessed spectrophotometrically.
RESULTS:After stimulation by serum replacement for 2 days, lipid droplets were accumulated surrounding nucleus of OP9 cells. When APS of 0.15-0.6 mM was administered without hyperthermia, the amount of lipid droplets was markedly suppressed to 50.5%∼-11.3% versus the undifferentiated control, and diminished huge aggregates of lipid droplets. In OP9 cells treated by hyperthermia at 42°C for 0.5 min, 1 min or 3 min in the absence of APS, adipogenesis was suppressed abruptly in a time-dependent manner to 95.4%, 18.7% or -5.5%, respectively. Whereas, the percentage of adipogenesis was 96.8% in OP9 cells treated by mild hyperthermia alone at 41°C for 1 min. The simultaneous application of APS and hyperthermia at 41°C for 1 min markedly suppressed the accumulation of lipid droplets to 25.7%∼-66.2%. By scanning electron microscopy (SEM) observation, the surface of OP9 cells treated with APS and hyperthermia appeared to have the morphological property of undifferentiated OP9 cells.
CONCLUSION:Combined treatment of APS and mild hyperthermia suppresses adipogenesis in OP9 cells, particularly in lipid droplets accumulation during spontaneous differentiation of OP9 preadipocytes.
巻・号 29(1)
ページ 30-7
公開日 2013-1-1
DOI 10.3109/02656736.2012.750016
PMID 23286696
MeSH Adipocytes / cytology Adipocytes / drug effects Adipocytes / metabolism Adipogenesis / drug effects* Animals Antineoplastic Agents / administration & dosage Antioxidants / administration & dosage* Ascorbic Acid / administration & dosage Ascorbic Acid / analogs & derivatives* Cell Line Hyperthermia, Induced* Lipid Metabolism / drug effects Mice Stromal Cells
IF 3.574
リソース情報
ヒト・動物細胞 OP9(RCB1124)