RRC ID 62260
Author Cortes-Dericks L, Froment L, Kocher G, Schmid RA.
Title Human lung-derived mesenchymal stem cell-conditioned medium exerts in vitro antitumor effects in malignant pleural mesothelioma cell lines.
Journal Stem Cell Res Ther
Abstract BACKGROUND:The soluble factors secreted by mesenchymal stem cells are thought to either support or inhibit tumor growth. Herein, we investigated whether the human lung-derived mesenchymal stem cell-conditioned medium (hlMSC-CM) exerts antitumor activity in malignant pleural mesothelioma cell lines H28, H2052 and Meso4.
METHODS:hlMSC-CM was collected from the human lung-derived mesenchymal stem cells. Inhibition of tumor cell growth was based on the reduction of cell viability and inhibition of cell proliferation using the XTT and BrdU assays, respectively. Elimination of tumor spheroids was assessed by the anchorage-independent sphere formation assay. The cytokine profile of hlMSC-CM was determined by a chemiluminescence-based cytokine array.
RESULTS:Our data showed that hlMSC-CM contains a broad range of soluble factors which include: cytokines, chemokines, hormones, growth and angiogenic factors, matrix metalloproteinases, metalloproteinase inhibitors and cell-cell mediator proteins. The 48- and 72-hour hlMSC-CM treatments of H28, H2052 and Meso4 cell lines elicited significant decreases in cell viability and inhibited cell proliferation. The 72-hour hlMSC-CM incubation of H28 cells completely eliminated the drug-resistant sphere-forming cells, which is more potent than twice the half maximal inhibitory concentration of cisplatin.
CONCLUSIONS:Our findings indicate that the cell-free hlMSC-CM confers in vitro antitumor activities via soluble factors in the tested mesothelioma cells and, hence, may serve as a therapeutic tool to augment the current treatment strategies in malignant pleural mesothelioma.
Volume 7
Pages 25
Published 2016-2-9
DOI 10.1186/s13287-016-0282-7
PII 10.1186/s13287-016-0282-7
PMID 26861734
PMC PMC4748521
MeSH Antineoplastic Agents / pharmacology* Cell Line, Tumor Cell Proliferation Cell Survival / drug effects Culture Media, Conditioned / chemistry Drug Screening Assays, Antitumor Humans Inhibitory Concentration 50 Lung / pathology Lung Neoplasms / drug therapy* Mesenchymal Stem Cells / metabolism* Mesothelioma / drug therapy* Mesothelioma, Malignant Pleural Neoplasms / drug therapy*
IF 5.116
Human and Animal Cells ACC-MESO-4(RCB2293)