論文 - 詳細
| RRC ID | 62267 |
|---|---|
| 著者 | Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Nishitsuji H, Ujino S, Shimotohno K, Iwamoto M, Watashi K, Wakita T, Watanabe M. |
| タイトル | Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus. |
| ジャーナル | Sci Rep |
| Abstract |
Hepatitis B virus (HBV) is not eradicated by current antiviral therapies due to persistence of HBV covalently closed circular DNA (cccDNA) in host cells, and thus development of novel culture models for productive HBV infection is urgently needed, which will allow the study of HBV cccDNA eradication. To meet this need, we developed culture models of HBV infection using human induced pluripotent stem cell-derived hepatocyte lineages, including immature proliferating hepatic progenitor-like cell lines (iPS-HPCs) and differentiated hepatocyte-like cells (iPS-Heps). These cells were susceptible to HBV infection, produced HBV particles, and maintained innate immune responses. The infection efficiency of HBV in iPS-HPCs predominantly depended on the expression levels of sodium taurocholate cotransporting polypeptide (NTCP), and was low relative to iPS-Heps: however, long-term culture of iPS-Heps was difficult. To provide a model for HBV persistence, iPS-HPCs overexpressing NTCP were established. The long-term persistence of HBV cccDNA was detected in iPS-HPCs overexpressing NTCP, and depended on the inhibition of the Janus-kinase signaling pathway. In conclusion, this study provides evidence that iPS-derived hepatic cell lines can be utilized for novel HBV culture models with genetic variation to investigate the interactions between HBV and host cells and the development of anti-HBV strategies. |
| 巻・号 | 6 |
| ページ | 29358 |
| 公開日 | 2016-7-8 |
| DOI | 10.1038/srep29358 |
| PII | srep29358 |
| PMID | 27386799 |
| PMC | PMC4937433 |
| MeSH | Cell Differentiation Cell Line Cell Proliferation DNA, Circular / genetics* DNA, Circular / immunology DNA, Viral / genetics DNA, Viral / immunology Hep G2 Cells Hepatitis B / genetics Hepatitis B / immunology Hepatitis B / metabolism Hepatitis B / virology* Hepatitis B virus / genetics Hepatitis B virus / immunology Hepatitis B virus / physiology* Hepatocytes / cytology* Hepatocytes / immunology Hepatocytes / metabolism Hepatocytes / virology Humans Immunity, Innate Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / immunology Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / virology MAP Kinase Signaling System Models, Biological* Organic Anion Transporters, Sodium-Dependent / genetics Organic Anion Transporters, Sodium-Dependent / metabolism* Symporters / genetics Symporters / metabolism* Transfection Virus Replication |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HiPS-RIKEN-2F(HPS0014) |