RRC ID 62277
著者 Sasaki A, Hiwatashi K, Kumagai M, Hata K, Kobayashi M.
タイトル Relationships between the expression of hepatocyte nuclear factors and factors essential for lipoprotein production in a human mesenchymal stem cell line, UE7T-13.
ジャーナル Biosci Biotechnol Biochem
Abstract To clarify the mechanisms regulating lipoprotein production by hepatocyte nuclear factors (HNFs), we generated four kinds of transfectants in human bone marrow mesenchymal stem cells: UE7T-13, stably expressing FOXA2 (also known as HNF3β), HNF4α, HNF1α or co-expressing HNF4α, and HNF1α (HNF4α/HNF1α). In HNF4α/HNF1α transfectants, cellular contents of triglycerides (TG) and cholesterol were markedly higher than in UE7T-13 cells and comparable to those in human hepatoma HepG2 cells. However, TG and cholesterol, which are secreted from cells as components of lipoproteins, were hardly detected in the medium for any of the transfectants. ApoB100 and MTP, which are essential for the formation and secretion of lipoproteins, were undetectable and detected at low levels, respectively, in HNF4α/HNF1α transfectants. We suggest that enforced co-expression of HNF4α and HNF1α is effective for cellular lipid accumulation, while additional factors are probably required for lipoprotein formation and secretion.
巻・号 81(2)
ページ 262-270
公開日 2017-2-1
DOI 10.1080/09168451.2016.1254530
PMID 27838959
MeSH Cell Differentiation Gene Expression Regulation* Hep G2 Cells Hepatocyte Nuclear Factor 1-alpha / genetics* Hepatocyte Nuclear Factor 3-beta / genetics Hepatocyte Nuclear Factor 4 / genetics Hepatocytes / cytology Hepatocytes / metabolism Humans Lipoproteins / biosynthesis* Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism*
IF 1.516
リソース情報
ヒト・動物細胞 UE7T-13(RCB2161) Hep G2