Reference - Detail
| RRC ID | 62304 |
|---|---|
| Author | Vinjamur DS, Bauer DE. |
| Title | Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines. |
| Journal | Methods Mol Biol |
| Abstract |
The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed. |
| Volume | 1698 |
| Pages | 275-284 |
| Published | 2018-1-1 |
| DOI | 10.1007/978-1-4939-7428-3_17 |
| PMID | 29076097 |
| MeSH | Biomarkers CRISPR-Cas Systems Cell Culture Techniques* Cell Differentiation Cell Line Cell Self Renewal Erythroid Precursor Cells / cytology* Erythroid Precursor Cells / metabolism* Fetal Blood / cytology* Flow Cytometry Genetic Engineering* / methods Genetic Vectors / genetics Hemoglobins / genetics Histocytochemistry Humans Karyotype Lentivirus / genetics Transduction, Genetic |
| Resource | |
| Human and Animal Cells | HUDEP-2(RCB4557) HUDEP-1(RCB4556) HUDEP-3(RCB4562) |