論文 - 詳細
| RRC ID | 62307 |
|---|---|
| 著者 | Kubota T, Hasuike A, Tsukune N, Ozawa Y, Yamamoto T, Min S, Naito M, Sato S. |
| タイトル | Influence of estrogen deficiency on guided bone augmentation: investigation of rat calvarial model and osteoblast-like MC3T3-E1 cells. |
| ジャーナル | Eur J Oral Sci |
| Abstract |
The effect of estrogen deficiency in bone augmentation, and the mechanisms by which estrogen deficiency impedes osteoblast differentiation and collagen matrix production, were examined. Twenty female Jcl:Wistar rats were divided into two groups: ovariectomized rats; and control rats. Guided bone augmentation was performed by positioning plastic caps in the calvarium of all animals at 8 wk after ovariectomy or sham surgery. Micro-computed tomography and histological sections were used to determine the amount of bone augmentation within the plastic caps. At 8 wk, there was statistically significantly less newly formed bone volume in ovariectomized rats. Immunohistological staining revealed the rare alignment of runt-related transcription factor 2-positive osteoblast-like cells and collagen I-positive bundle fibers in ovariectomized rats. In cell culture experiments, pre-osteoblast-like cells, MC3T3-E1, were treated with the estrogen receptor antagonist, fulvestrant. In treated cells, alkaline phosphatase activity remained high, whereas Alizarin Red staining was completely inhibited. Extracellular staining intensity of collagen I was decreased after fulvestrant treatment. Consistent with these observations, gene-expression analysis confirmed that fulvestrant treatment led to weaker expression of mRNA for osteogenic transcription factors and bone matrix protein-related genes. The results demonstrate that estrogen deficiency suppresses osteoblast differentiation and collagen matrix production in bone augmentation. |
| 巻・号 | 126(3) |
| ページ | 206-213 |
| 公開日 | 2018-6-1 |
| DOI | 10.1111/eos.12415 |
| PMID | 29676477 |
| MeSH | Animals Bone Regeneration / physiology* Cell Differentiation Core Binding Factor Alpha 1 Subunit Estrogens / deficiency* Female Guided Tissue Regeneration* Osteoblasts / cytology Osteoblasts / physiology* Ovariectomy RNA, Messenger / metabolism Rats Rats, Wistar X-Ray Microtomography |
| IF | 2.22 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |