| RRC ID |
62332
|
| Author |
Nakagawa T, Fujita N, Oh-Hara T, Kurokawa T, Nakamura K, Tsuruo T.
|
| Title |
Interleukin-1 alpha induced cyclooxygenase-2 expression in bone-derived endothelial cells.
|
| Journal |
J Cell Physiol
|
| Abstract |
Histological studies have suggested that vascular endothelial cells in bone are members of a complex network that regulates bone development and remodeling by producing soluble factors or by mediating cell-cell adhesion. To clarify the role of bone-derived endothelial cell lines (BDECs) in bone remodeling, we established several clones of BDECs from the femurs of BALB/c mice after transformation with the SV40 virus. Then we examined the response of these clones to interleukin-1alpha (IL-1alpha). IL-1alpha is known to induce bone resorption in part by increasing the expression of cyclooxygenase-2 (COX-2) that is associated with the production of PGE2 in osteoblast-lineage cells. Treating the primary and established BDECs with IL-1alpha induced COX-2 mRNA expression. A transcriptional activation assay revealed that the treatment with IL-1alpha increased COX-2 promoter activity in a dose-dependent manner, and IL-1alpha promoted COX-2 protein expression in BDECs. Treatment with IL-1alpha promoted PGE2 production from BDECs in a dose-dependent manner. These results indicate that IL-1alpha stimulates PGE2 synthesis largely by inducing BDECs to express COX-2. Because PGE2 stimulates bone resorption, these vascular endothelial cells, as well as osteoblast cells, play important roles in bone remodeling.
|
| Volume |
179(2)
|
| Pages |
226-32
|
| Published |
1999-5-1
|
| DOI |
10.1002/(SICI)1097-4652(199905)179:2<226::AID-JCP13>3.0.CO;2-Q
|
| PII |
10.1002/(SICI)1097-4652(199905)179:2<226::AID-JCP13>3.0.CO;2-Q
|
| PMID |
10199562
|
| MeSH |
Animals
Bone Remodeling / drug effects*
Cell Line
Cyclooxygenase 2
Dinoprostone / metabolism
Endothelium, Vascular / drug effects*
Femur / metabolism
Gene Expression Regulation, Enzymologic / drug effects
Interleukin-1 / pharmacology*
Isoenzymes / biosynthesis*
Mice
Mice, Inbred BALB C
Prostaglandin-Endoperoxide Synthases / biosynthesis*
RNA, Messenger / metabolism
Receptors, Interleukin-1 / drug effects
Transfection
Transformation, Genetic
|
| IF |
5.546
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
