RRC ID 6242
Author Kanungo J, Zheng YL, Amin ND, Kaur S, Ramchandran R, Pant HC.
Title Specific inhibition of cyclin-dependent kinase 5 activity induces motor neuron development in vivo.
Journal Biochem Biophys Res Commun
Abstract Cyclin-dependent kinase 5 (cdk5) is a ubiquitous protein activated by specific activators, p35 and p39. Cdk5 regulates neuronal migration, differentiation, axonogenesis, synaptic transmission and apoptosis. However, its role in motor neuron development remains unexplored. Here, using gain and loss-of-function analyses in developing zebrafish embryos, we report that cdk5 plays a critical role in spinal and cranial motor neuron development. Cdk5 knockdown results in supernumerary spinal and cranial motor neurons. While a dominant negative, kinase-dead cdk5 promotes the generation of supernumerary motor neurons; over-expression of cdk5 suppresses motor neuron development. Thus, modulating cdk5 activity seems promising in inducing motor neuron development in vivo.
Volume 386(1)
Pages 263-7
Published 2009-8-14
DOI 10.1016/j.bbrc.2009.06.038
PII S0006-291X(09)01172-3
PMID 19523926
PMC PMC2758157
MeSH Animals Brain / cytology Brain / embryology Cyclin-Dependent Kinase 5 / antagonists & inhibitors Cyclin-Dependent Kinase 5 / genetics* Embryo, Nonmammalian / physiology Gene Knockdown Techniques Motor Neurons / enzymology Motor Neurons / physiology* Neurogenesis / genetics* Oligonucleotides, Antisense / genetics Spinal Cord / cytology Spinal Cord / embryology Zebrafish / embryology* Zebrafish / genetics*
IF 2.705
Times Cited 6
Zebrafish rw0(Tg(CM-isl1:GFP))