| RRC ID |
62630
|
| 著者 |
Germond A, Panina Y, Shiga M, Niioka H, Watanabe TM.
|
| タイトル |
Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy.
|
| ジャーナル |
Anal Chem
|
| Abstract |
Monitoring cell-state transition in pluripotent cells is invaluable for application and basic research. In this study, we demonstrate the pertinence of noninvasive, label-free Raman spectroscopy to monitor and characterize the cell-state transition of mouse stem cells undergoing reprogramming. Using an isogenic cell line of mouse stem cells, reprogramming from neuronal cells was performed, and we showcase a comparative analysis of living single-cell spectral data of the original stem cells, their neuronal progenitors, and reprogrammed cells. Neural network, regression models, and ratiometric analyses were used to discriminate the cell states and extract several important biomarkers specific to differentiation or reprogramming. Our results indicated that the Raman spectrum allowed us to build a low-dimensional space allowing us to monitor and characterize the dynamics of cell-state transition at a single-cell level, scattered in heterogeneous populations. The ability of monitoring pluripotency by Raman spectroscopy and distinguishing differences between ES and reprogrammed cells is also discussed.
|
| 巻・号 |
92(22)
|
| ページ |
14915-14923
|
| 公開日 |
2020-11-17
|
| DOI |
10.1021/acs.analchem.0c01800
|
| PMID |
33112148
|
| MeSH |
Animals
Biomarkers / metabolism
Cellular Reprogramming*
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / metabolism
Mice
Spectrum Analysis, Raman*
|
| IF |
6.785
|
| リソース情報 |
| ヒト・動物細胞 |
EB5(AES0151) |
