論文 - 詳細
| RRC ID | 62693 |
|---|---|
| 著者 | Kitazawa Y, Ueta H, Sawanobori Y, Katakai T, Yoneyama H, Ueha S, Matsushima K, Tokuda N, Matsuno K. |
| タイトル | Novel Targeting to XCR1+ Dendritic Cells Using Allogeneic T Cells for Polytopical Antibody Responses in the Lymph Nodes. |
| ジャーナル | Front Immunol |
| Abstract |
Vaccination strategy that induce efficient antibody responses polytopically in most lymph nodes (LNs) against infections has not been established yet. Because donor-specific blood transfusion induces anti-donor class I MHC antibody production in splenectomized rats, we examined the mechanism and significance of this response. Among the donor blood components, T cells were the most efficient immunogens, inducing recipient T cell and B cell proliferative responses not only in the spleen, but also in the peripheral and gut LNs. Donor T cells soon migrated to the splenic T cell area and the LNs, with a temporary significant increase in recipient NK cells. XCR1+ resident dendritic cells (DCs), but not XCR1- DCs, selectively phagocytosed donor class I MHC+ fragments after 1 day. After 1.5 days, both DC subsets formed clusters with recipient CD4+ T cells, which proliferated within these clusters. Inhibition of donor T cell migration or depletion of NK cells by pretreatment with pertussis toxin or anti-asialoGM1 antibody, respectively, significantly suppressed DC phagocytosis and subsequent immune responses. Three allogeneic strains with different NK activities had the same response but with different intensity. Donor T cell proliferation was not required, indicating that the graft vs. host reaction is dispensable. Intravenous transfer of antigen-labeled and mitotic inhibitor-treated allogeneic, but not syngeneic, T cells induced a polytopical antibody response to labeled antigens in the LNs of splenectomized rats. These results demonstrate a novel mechanism of alloresponses polytopically in the secondary lymphoid organs (SLOs) induced by allogeneic T cells. Donor T cells behave as self-migratory antigen ferries to be delivered to resident XCR1+ DCs with negligible commitment of migratory DCs. Allogeneic T cells may be clinically applicable as vaccine vectors for polytopical prophylactic antibody production even in asplenic or hyposplenic individuals. |
| 巻・号 | 10 |
| ページ | 1195 |
| 公開日 | 2019-1-1 |
| DOI | 10.3389/fimmu.2019.01195 |
| PMID | 31191552 |
| PMC | PMC6548820 |
| MeSH | Animals Blood Donors Blood Transfusion Cell Movement Dendritic Cells / chemistry Dendritic Cells / immunology* Epitopes / immunology G(M1) Ganglioside / immunology G(M1) Ganglioside / pharmacology Histocompatibility Antigens Class I / immunology* Isoantibodies / biosynthesis* Isoantibodies / immunology Killer Cells, Natural / immunology Lymph Nodes / immunology* Lymphocyte Activation Lymphocyte Transfusion Pertussis Toxin / immunology Pertussis Toxin / pharmacology Peyer's Patches / immunology Phagocytosis Rats Rats, Inbred ACI Rats, Inbred Lew Receptors, G-Protein-Coupled / analysis* Spleen / immunology Splenectomy T-Lymphocytes / immunology* |
| IF | 5.085 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | ACI/NKyo (StrainID=6) |