RRC ID 62695
著者 Ueta H, Xu XD, Yu B, Kitazawa Y, Yu E, Hara Y, Morita-Nakagawa M, Zhou S, Sawanobori Y, Ueha S, Rokutan K, Tanaka T, Tokuda N, Matsushima K, Matsuno K.
タイトル Suppression of liver transplant rejection by anti-donor MHC antibodies via depletion of donor immunogenic dendritic cells.
ジャーナル Int Immunol
Abstract BACKGROUND:We previously found two distinct passenger dendritic cell (DC) subsets in the rat liver that played a central role in the liver transplant rejection. In addition, tolerance-inducing protocol, donor-specific transfusion (DST), triggered systemic polytopical production of depleting alloantibodies to donor class I MHC antigen (DST-antibodies).
METHODS:We examined the role of DST-antibodies in the trafficking of graft DC subsets and the alloresponses in a rat model. We also examined an anti-donor class II MHC (MHCII) antibody that recognizes donor DCs more selectively.
RESULTS:Preoperative transfer of DST-antibodies and DST pretreatments eliminated all passenger leukocytes, including both DC subsets and depleted the sessile DCs in the graft to ~20% of control. The CD172a +CD11b/c + immunogenic subset was almost abolished. The intrahost direct or semi-direct allorecognition pathway was successfully blocked, leading to a significant suppression of the CD8 + T-cell response in the recipient lymphoid organs and the graft with delayed graft rejection. Anti-donor MHCII antibody had similar effects without temporary graft damage. Although DST pretreatment had a priming effect on the recipient Treg proliferative response, DST-primed sera and the anti-donor MHCII antibody did not.
CONCLUSION:DST-antibodies and anti-donor MHCII antibodies could suppress the CD8 + T-cell-mediated liver transplant rejection by depleting donor immunogenic DCs, blocking the direct or semi-direct pathway of allorecognition. Donor MHCII-specific antibodies may be applicable as a selective suppressant of anti-donor immunity for clinical liver transplantation without the cellular damage of donor MHCII - graft cells and recipient cells.
巻・号 33(5)
ページ 261-272
公開日 2021-4-22
DOI 10.1093/intimm/dxaa076
PII 6015059
PMID 33258927
PMC PMC8060989
MeSH Animals Animals, Genetically Modified / immunology Antibody Formation / immunology Antigens, Differentiation / immunology CD11b Antigen / immunology CD8-Positive T-Lymphocytes Dendritic Cells / immunology* Graft Rejection / immunology* Graft Survival / immunology Histocompatibility Antigens Class I / immunology* Immune Tolerance / immunology Isoantibodies / immunology* Liver Transplantation / methods Rats Rats, Inbred Lew T-Lymphocytes, Regulatory / immunology Tissue Donors Transplantation, Homologous / methods
IF 3.519
リソース情報
ラット ACI/NKyo (StrainID=6)