| RRC ID |
62698
|
| 著者 |
Yasuda N, Sasaki M, Kataoka-Sasaki Y, Nagahama H, Kocsis JD, Kawaharada N, Honmou O.
|
| タイトル |
Intravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia.
|
| ジャーナル |
Brain Res
|
| Abstract |
Ischemic spinal cord injury (iSCI) is a devastating complication of aortic surgery, with few strategies for prevention. Intravenous infusion of mesenchymal stem cells (MSCs) for iSCI has been shown to provide functional improvement through protection of gray matter. The purpose of this study was to investigate additional mechanisms which may exert therapeutic efficacy in iSCI. Severe iSCI was created to occlude the descending aorta, which was cross-clamped 5 mm distal to the left subclavian artery for 16 min. One day after iSCI induction, iSCI rats were randomized into two groups: one received intravenous infusion of MSCs (MSC-group), the other received vehicle (no cells; vehicle-group). Locomotor function and in vivo MRI were recorded. H&E, Nissl and toluidine blue stainings, immunohistochemical analysis, diffusion tensor imaging (DTI), and the assessment of blood-spinal cord barrier (BSCB) stability were performed. MSC treated animals exhibited gradual improvement in hind-limb locomotor function during the 4-week study period; however the vehicle-treated group displayed persistent motor deficits. In the MSC-treated group we observed the protection of white and gray matter volume reduction of axonal and neuronal loss or degeneration and preservation of microvasculature including BSCB function. Intravenous infusion of MSCs may provide therapeutic efficacy to improve functional outcomes in a rat model of severe iSCI via protection of white and gray matter.
|
| 巻・号 |
1747
|
| ページ |
147040
|
| 公開日 |
2020-11-15
|
| DOI |
10.1016/j.brainres.2020.147040
|
| PII |
S0006-8993(20)30398-X
|
| PMID |
32771405
|
| MeSH |
Administration, Intravenous
Animals
Disease Models, Animal
Gray Matter / diagnostic imaging
Gray Matter / pathology*
Magnetic Resonance Imaging
Mesenchymal Stem Cells*
Motor Activity / physiology*
Rats
Spinal Cord Ischemia / diagnostic imaging
Spinal Cord Ischemia / pathology
Spinal Cord Ischemia / therapy*
White Matter / diagnostic imaging
White Matter / pathology*
|
| IF |
2.733
|
| リソース情報 |
| ラット |
W-Tg(CAG-GFP)184Ys (StrainID=525) |
