RRC ID 62707
Author Takeda Y, Murata T, Jamsransuren D, Suganuma K, Kazami Y, Batkhuu J, Badral D, Ogawa H.
Title Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2.
Journal Viruses
Abstract Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), and norovirus (NV) are highly contagious pathogens that threaten human health. Here we focused on the antiviral potential of the medicinal herb, Saxifraga spinulosa (SS). Water-soluble extracts of SS were prepared, and their virus-inactivating activity was evaluated against the human virus pathogens SARS-CoV-2 and IAV; we also examined virucidal activity against feline calicivirus and murine norovirus, which are surrogates for human NV. Among our findings, we found that SS-derived gallocatechin gallate compounds were capable of inactivating all viruses tested. Interestingly, a pyrogallol-enriched fraction (Fr 1C) inactivated all viruses more rapidly and effectively than did any of the component compounds used alone. We found that 25 µg/mL of Fr 1C inactivated >99.6% of SARS-CoV-2 within 10 s (reduction of ≥2.33 log10 TCID50/mL). Fr 1C resulted in the disruption of viral genomes and proteins as determined by gel electrophoresis, electron microscopy, and reverse transcription-PCR. Taken together, our results reveal the potential of Fr 1C for development as a novel antiviral disinfectant.
Volume 12(7)
Published 2020-6-28
DOI 10.3390/v12070699
PII v12070699
PMID 32605306
PMC PMC7411974
MeSH Antiviral Agents / pharmacology* Betacoronavirus / drug effects* Betacoronavirus / ultrastructure Calicivirus, Feline / drug effects Electrophoresis, Polyacrylamide Gel Genome, Viral / drug effects Hemagglutination Tests Humans Influenza A virus / drug effects* Microscopy, Electron, Transmission Norovirus / drug effects* Plant Extracts / pharmacology* Plants, Medicinal* Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 Saxifragaceae* Viral Proteins / drug effects
IF 3.816
Human and Animal Cells RAW 264(RCB0535)