RRC ID 62718
著者 Tani R, Hayakawa K, Tanaka S, Shiota K.
タイトル Linker histone variant H1T targets rDNA repeats.
ジャーナル Epigenetics
Abstract H1T is a linker histone H1 variant that is highly expressed at the primary spermatocyte stage through to the early spermatid stage of spermatogenesis. While the functions of the somatic types of H1 have been extensively investigated, the intracellular role of H1T is unclear. H1 variants specifically expressed in germ cells show low amino acid sequence homology to somatic H1s, which suggests that the functions or target loci of germ cell-specific H1T differ from those of somatic H1s. Here, we describe the target loci and function of H1T. H1T was expressed not only in the testis but also in tumor cell lines, mouse embryonic stem cells (mESCs), and some normal somatic cells. To elucidate the intracellular localization and target loci of H1T, fluorescent immunostaining and ChIP-seq were performed in tumor cells and mESCs. We found that H1T accumulated in nucleoli and predominantly targeted rDNA repeats, which differ from somatic H1 targets. Furthermore, by nuclease sensitivity assay and RT-qPCR, we showed that H1T repressed rDNA transcription by condensing chromatin structure. Imaging analysis indicated that H1T expression affected nucleolar formation. We concluded that H1T plays a role in rDNA transcription, by distinctively targeting rDNA repeats.
巻・号 11(4)
ページ 288-302
公開日 2016-4-2
DOI 10.1080/15592294.2016.1159369
PMID 27018843
PMC PMC4889250
MeSH Amino Acid Sequence / genetics Animals Cell Nucleolus / genetics Chromatin / genetics DNA, Ribosomal / biosynthesis DNA, Ribosomal / genetics* Gene Expression Regulation, Developmental Germ Cells / metabolism Histones / biosynthesis Histones / genetics* Male Mice Mouse Embryonic Stem Cells / metabolism Sequence Homology, Amino Acid Spermatids / growth & development Spermatids / metabolism Spermatocytes / growth & development Spermatocytes / metabolism Spermatogenesis / genetics* Testis / growth & development Testis / metabolism Transcription, Genetic*
IF 4.251
リソース情報
遺伝子材料 pPyCAG-BstXI-IB (RDB12107)
ヒト・動物細胞 Kato III(RCB2088)