| RRC ID |
62742
|
| 著者 |
Nakayama A, Aoki S, Uchihashi K, Nishijima-Matsunobu A, Yamamoto M, Kakihara N, Iwakiri R, Fujimoto K, Toda S.
|
| タイトル |
Interaction between Esophageal Squamous Cell Carcinoma and Adipose Tissue in Vitro.
|
| ジャーナル |
Am J Pathol
|
| Abstract |
Esophageal squamous cell carcinoma (ESCC) develops within the squamous epithelial layer and invades the submucosa to the subadventitia that has adipose tissue (AT). AT seems critical to ESCC progression, but the underlying mechanism is unknown. We aimed to address the association between ESCC and AT in vitro. ESCC cells were cultured on rat or human subcutaneous AT-embedded or -non-embedded collagen gel. AT promoted the growth of ESCC cells and inhibited their apoptosis. AT promoted the expression of the squamous differentiation marker involucrin in ESCC cells. AT accelerated the expression of invasion-related factors in poorly differentiated ESCC cells only. AT promoted the expression of phosphorylated-insulin-like growth factor-1 receptor in ESCC cells, whereas it inhibited that of the human epidermal growth factor receptor 2. Insulin-like growth factor-1, but not leptin, adiponectin, or resistin, promoted and inhibited the growth and apoptosis of ESCC cells, respectively. In turn, ESCC cells decreased the production of these adipokines in AT and the number of preadipocytes and mesenchymal stem cell-like cells, which developed from AT. These results suggest that i) AT may influence the progression of ESCC with increased growth or invasion and decreased apoptosis through insulin-like growth factor-1/insulin-like growth factor-1 receptor signaling, ii) AT may affect human epidermal growth factor receptor 2-targeted therapy; and iii) the cancer cells may affect adipokine production in AT.
|
| 巻・号 |
186(5)
|
| ページ |
1180-94
|
| 公開日 |
2016-5-1
|
| DOI |
10.1016/j.ajpath.2016.01.003
|
| PII |
S0002-9440(16)00079-1
|
| PMID |
26952643
|
| MeSH |
Adiponectin / pharmacology
Adipose Tissue / physiology*
Animals
Apoptosis / physiology
Biomarkers, Tumor / metabolism
Carcinoma, Squamous Cell / physiopathology*
Cell Adhesion Molecules / metabolism
Cell Line, Tumor
Cell Transformation, Neoplastic
Esophageal Neoplasms / physiopathology*
Esophageal Squamous Cell Carcinoma
Filamins / metabolism
Humans
Hypertrophy / physiopathology
Insulin-Like Growth Factor I
Lipid Metabolism / physiology
Matrix Metalloproteinase 14 / metabolism
Matrix Metalloproteinase 9 / metabolism
Microscopy, Electron
Protein Precursors / metabolism
Rats, Wistar
Receptor, ErbB-2 / metabolism
Resistin / pharmacology
Stromal Cells / physiology
Tumor Cells, Cultured
|
| IF |
3.491
|
| リソース情報 |
| ヒト・動物細胞 |
EC-GI-10(RCB0774)
TE-9(RCB1988) |
