RRC ID 62761
著者 Terada D, Yokoyama Y, Hattori S, Kobayashi H, Tamada Y.
タイトル The outermost surface properties of silk fibroin films reflect ethanol-treatment conditions used in biomaterial preparation.
ジャーナル Mater Sci Eng C Mater Biol Appl
Abstract Silk fibroin has attracted interest as a biomaterial, given its many excellent properties. Cell attachment to silk substrates is usually weaker than to standard culture dishes, and cells cultured on silk films or hydrogels typically form spheroids and micro-aggregates. However, too little is known about the higher order structures and behavior of fibroin under different conditions to explain the features of silk fibroin as a culture substrate. For instance, different biomaterial surfaces, with distinct effects on cell culture, can be achieved by varying the conditions of crystallization by alcohol immersion. Here, we show that treatment of fibroin film with <80% ethanol results in a jelly-like, hydrated hydrogel as the outermost surface layer; fibroblasts preferably aggregate, rather than attach individually to such a hydrogel surface, and therefore aggregate into spheroids. In contrast, a fibroin film treated with >90% ethanol has a harder surface than the <80% ethanol-treated fibroin, to which individual cells prefer to attach (and then expand on the surface), rather than to aggregate. We discuss the influence of alcohol concentration on the surface properties, based on surface analysis of the films. The surface analysis involved assessment of static and dynamic contact angles, zeta potential, changes in crystallinity and microscopic morphology of electrospun fibers, and texture changes of the outermost surface at a nanometer-scale captured by a scanning probe microscope.
巻・号 58
ページ 119-26
公開日 2016-1-1
DOI 10.1016/j.msec.2015.07.041
PII S0928-4931(15)30230-7
PMID 26478294
MeSH Animals Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Cell Adhesion / drug effects Ethanol / chemistry* Fibroins / chemistry* Mice NIH 3T3 Cells Silk / chemistry* Surface Properties
IF 5.88
リソース情報
ヒト・動物細胞 NIH3T3